Trypanosoma cruzi: amastigote polymorphism defined by monoclonal antibodies

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dc.contributor.authorVerbisck, Newton Valério [UNIFESP]
dc.contributor.authorDa-Silva, S. [UNIFESP]
dc.contributor.authorMortara, Renato Arruda [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2015-06-14T13:24:48Z
dc.date.available2015-06-14T13:24:48Z
dc.date.issued1998-12-01
dc.description.abstractWe have raised monoclonal antibodies (mAbs) directed towards amastigote forms of Trypanosoma cruzi, and shown that mAbs 1D9 and 4B9 are carbohydrate while mAb 4B5 activity is resistant to periodate oxidation of the antigen. Here we used an ELISA to quantitate and compare the expression of surface epitopes on fixed parasites among different parasite isolates. The expression of markers varied among T. cruzi amastigotes isolated from infected cells or after extracellular differentiation of trypomastigotes. Moreover, we also observed an extensive polymorphic expression of these epitopes among amastigotes derived from different strains and clones. For instance, mAb 2C2 strongly and evenly reacted with 9 strains and clones (G, Y, CL, Tulahuen, MD, and F, and clones Sylvio X-10/4, D11, and CL.B), with absorbance at 492 nm (A492 nm) from 0.6 to 0.8. By contrast, mAb 4B5 had a higher expression in Tulahuen amastigotes (around 0.9 at 492 nm) whereas its reactivity with amastigotes from clones CL.B, Sylvio X-10/4 and D11 was much lower (around 0.4). mAb 1D9 displayed an interesting pattern of reactivity with amastigotes of the different strains and clones (A492 nm of G>D11³Sylvio X-10/4 = MD>Tulahuen = F = Y>CL>CL.B). Finally, we observed that mAb 4B9 had the lowest reaction with the parasites studied, with higher values of A492 nm with Y strain (around 0.6) and lower values with Tulahuen, F and CL.B strains (around 0.2). Immunoblotting analysis also showed extensive variations among amastigotes of the various parasite isolates and mAbs 4B9, 1D9 and 4B5 revealed significant differences in expression between clones and parental strains. These data describe a previously uncharacterized polymorphism of T. cruzi amastigote surface components.en
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP)
dc.description.affiliationUnifespUNIFESP, EPM
dc.description.sourceSciELO
dc.format.extent1583-1591
dc.identifierhttp://dx.doi.org/10.1590/S0100-879X1998001200011
dc.identifier.citationBrazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 31, n. 12, p. 1583-1591, 1998.
dc.identifier.doi10.1590/S0100-879X1998001200011
dc.identifier.fileS0100-879X1998001200011.pdf
dc.identifier.issn0100-879X
dc.identifier.scieloS0100-879X1998001200011
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/706
dc.identifier.wosWOS:000077232400011
dc.language.isoeng
dc.publisherAssociação Brasileira de Divulgação Científica
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectTrypanosoma cruzien
dc.subjectamastigoteen
dc.subjectmonoclonal antibodiesen
dc.subjectsurface antigenen
dc.subjectpolymorphismen
dc.titleTrypanosoma cruzi: amastigote polymorphism defined by monoclonal antibodiesen
dc.typeinfo:eu-repo/semantics/article
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