Role of Leishmania (Leishmania) amazonensis amastigote glycosphingolipids in macrophage infectivity

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dc.contributor.authorTanaka, Améria Kaori [UNIFESP]
dc.contributor.authorGorin, Philip Albert James
dc.contributor.authorTakahashi, Helio Kiyoshi [UNIFESP]
dc.contributor.authorStraus, Anita Hilda [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Federal do Paraná Departamento de Bioquímica
dc.date.accessioned2015-06-14T13:36:59Z
dc.date.available2015-06-14T13:36:59Z
dc.date.issued2007-06-01
dc.description.abstractThe role of glycosphingolipids (GSLs) present in amastigote forms of Leishmania (Leishmania) amazonensis during infection of macrophages was analyzed, with particular emphasis on GSLs presenting the terminal Galpß1-3Galpa disaccharide. Macrophage invasion by L. (L.) amazonensis amastigotes was reduced by 37% when the disaccharide Galpß1-3Galp (1 mM) was added to the culture medium. The putative macrophage receptor/lectin for ß-Gal-globotriaosylceramide (Galpß1-3Galpa1-4Galpß1-4Glc pß1-1Cer) and other structurally related GSLs from L. (L.) amazonensis amastigotes were analyzed by micelles and parasite binding assay to peritoneal macrophage proteins fractionated by SDS-PAGE under nonreducing conditions. Micelles containing purified amastigote GSLs or a suspention of L. (L.) amazonensis amastigotes fixed with 2% formaldehyde were incubated with nitrocellulose membrane containing the macrophage proteins transferred by Western blotting. Binding of micelles containing purified GSLs from amastigote forms or fixed L. (L.) amazonensis amastigotes to nitrocellulose membrane was probed using monoclonal antibody ST-3, which recognizes the glycoepitope Galpß1-3Galpa1-R present either in the micelle preparation or on the amastigote surface. Macrophage protein with molecular mass ~30 kDa bound the amastigote GSL and appeared to be a doublet on electrophoresis. The specificity of this interaction was confirmed using fixed L. (L.) chagasi amastigotes, which do not express GSLs such as ß-Galp-globotriaosylceramides, and which do not bind to 30-kDa protein.en
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de Bioquímica
dc.description.affiliationUniversidade Federal do Paraná Departamento de Bioquímica
dc.description.affiliationUnifespUNIFESP, EPM, Depto. de Bioquímica
dc.description.sourceSciELO
dc.format.extent799-806
dc.identifierhttp://dx.doi.org/10.1590/S0100-879X2006005000106
dc.identifier.citationBrazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 40, n. 6, p. 799-806, 2007.
dc.identifier.doi10.1590/S0100-879X2006005000106
dc.identifier.fileS0100-879X2007000600008.pdf
dc.identifier.issn0100-879X
dc.identifier.scieloS0100-879X2007000600008
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/3777
dc.identifier.wosWOS:000248409900008
dc.language.isoeng
dc.publisherAssociação Brasileira de Divulgação Científica
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectLeishmania (Leishmania) amazonensisen
dc.subjectAmastigoteen
dc.subjectGlycosphingolipiden
dc.subjectMacrophage binding proteinen
dc.titleRole of Leishmania (Leishmania) amazonensis amastigote glycosphingolipids in macrophage infectivityen
dc.typeinfo:eu-repo/semantics/article
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