Relative populations and toxin production by Aspergillus flavus and Fusarium verticillioides in artificially inoculated corn at various stages of development under field conditions

dc.contributor.authorZorzete, Patricia
dc.contributor.authorCastro, Rosimar S.
dc.contributor.authorPozzi, Claudia R.
dc.contributor.authorIsrael, Ana Lia M.
dc.contributor.authorFonseca, Hornero
dc.contributor.authorYanaguibashi, Gianni [UNIFESP]
dc.contributor.authorCorrea, Benedito
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionInst Zootecnia
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.description.abstractThe presence, development and production of mycotoxins by Aspergillus flavus and Fusarium verticillioides were studied in corn ears under field conditions after artificial contamination of corn silks. the planted area was divided into five treatments: T1, inoculated with A.flavus solution containing 1 x 10(8) spores, ears covered; T2, inoculated with F. verticillioides solution containing 1 X 10(8) spores, ears covered; T3, inoculated with E verticillioides plus A. flavus solution containing 1 x 10(8) spores of each, ears covered; T4, sprayed with sterile phosphate-buffered saline, ears covered; TS, non-sprayed silks, uncovered ears. Soil and air samples were also collected and analysed for the occurrence of fungi. Water activity, relative air humidity, rainfall and temperature were determined to assess the correlation between abiotic factors and the presence of fungi in the samples. Contamination with the inoculated fungus predominated in T1 and T2. in the other treatments, F. verticillioides was the most frequently isolated contaminant irrespective of treatment. Considering the production of mycotoxins, a positive relation between the production of fumonisins B-1 and B-2 and the frequency of F. verticillioides was statistically verified in all treatments. (C) 2007 Society of Chemical Industry.en
dc.description.affiliationUniv São Paulo, Inst Ciencias Biomed, Dept Microbiol, BR-05508900 São Paulo, Brazil
dc.description.affiliationInst Zootecnia, Nova Odessa, SP, Brazil
dc.description.affiliationUniv São Paulo, ESALQ, Dept Agr, Piracicaba, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, SEA, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Pró-Reitoria da Pós-graduação da Universidade Federal de São Paulo, UNIFESP/SEA, São Paulo, SP, Brazil, São Paulo
dc.description.sourceWeb of Science
dc.identifier.citationJournal of the Science of Food and Agriculture. Chichester: John Wiley & Sons Ltd, v. 88, n. 1, p. 48-55, 2008.
dc.relation.ispartofJournal of the Science of Food and Agriculture
dc.rightsAcesso restrito
dc.subjectFusarium verticillioidesen
dc.subjectAspergillus flavusen
dc.subjectabiotic factorsen
dc.titleRelative populations and toxin production by Aspergillus flavus and Fusarium verticillioides in artificially inoculated corn at various stages of development under field conditionsen