Multiple RNAs from the mouse carboxypeptidase M locus: functional RNAs or transcription noise?

Página do item simplificado
dc.contributor.authorGuimarães, Alessander de Oliveira [UNIFESP]
dc.contributor.authorMotta, Fabiana Louise Teixeira [UNIFESP]
dc.contributor.authorAlves, Viviane S. [UNIFESP]
dc.contributor.authorCastilho, Beatriz Amaral de [UNIFESP]
dc.contributor.authorPesquero, João Bosco [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T13:52:16Z
dc.date.available2016-01-24T13:52:16Z
dc.date.issued2009-02-08
dc.description.abstractBackground: A major effort of the scientific community has been to obtain complete pictures of the genomes of many organisms. This has been accomplished mainly by annotation of structural and functional elements in the genome sequence, a process that has been centred in the gene concept and, as a consequence, biased toward protein coding sequences. Recently, the explosion of transcriptome data generated and the discovery of many functional non-protein coding RNAs have painted a more detailed and complex scenario for the genome. Here we analyzed the mouse carboxypeptidase M locus in this broader perspective in order to define the mouse CPM gene structure and evaluate the existence of other transcripts from the same genomic region.Results: Bioinformatic analysis of nucleotide sequences that map to the mouse CPM locus suggests that, in addition to the mouse CPM mRNA, it expresses at least 33 different transcripts, many of which seem to be non-coding RNAs. We randomly chose to evaluate experimentally four of these extra transcripts. They are expressed in a tissue specific manner, indicating that they are not artefacts or transcriptional noise. Furthermore, one of these four extra transcripts shows expression patterns that differed considerably from the other ones and from the mouse CPM gene, suggesting that there may be more than one transcriptional unit in this locus. in addition, we have confirmed the mouse CPM gene RefSeq sequence by rapid amplification of cDNA ends (RACE) and directional cloning.Conclusion: This study supports the recent view that the majority of the genome is transcribed and that many of the resulting transcripts seem to be non-coding RNAs from introns of genes or from independent transcriptional units. Although some of the information on the transcriptome of many organisms may actually be artefacts or transcriptional noise, we argue that it can be experimentally evaluated and used to find and define biological functional elements on the genome. Furthermore, the transcription of other functional RNAs besides the protein coding RNA from a specific genomic locus imposes extra care when designing and interpreting experiments involving genetic manipulations or expression detection and quantification.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biofis, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Microbiol & Imunol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biofis, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Microbiol & Imunol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent14
dc.identifierhttp://dx.doi.org/10.1186/1471-2199-10-7
dc.identifier.citationBmc Molecular Biology. London: Biomed Central Ltd, v. 10, 14 p., 2009.
dc.identifier.doi10.1186/1471-2199-10-7
dc.identifier.fileWOS000264206600001.pdf
dc.identifier.issn1471-2199
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/31316
dc.identifier.wosWOS:000264206600001
dc.language.isoeng
dc.publisherBiomed Central Ltd
dc.relation.ispartofBmc Molecular Biology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleMultiple RNAs from the mouse carboxypeptidase M locus: functional RNAs or transcription noise?en
dc.typeinfo:eu-repo/semantics/article
Arquivos
Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
WOS000264206600001.pdf
Tamanho:
2.12 MB
Formato:
Adobe Portable Document Format
Descrição:
Baixar
Coleções
EPM - Artigos