High serum levels of soluble Fas (sFas) in CKD patients: Effects of renal clearance, reabsorption and synthesis

Página do item simplificado
dc.contributor.authorDalboni, Maria Aparecida [UNIFESP]
dc.contributor.authorCenedeze, Marcos Antonio [UNIFESP]
dc.contributor.authorManfredi, Silvia Regina [UNIFESP]
dc.contributor.authorAndreoli, Maria Claudia Cruz [UNIFESP]
dc.contributor.authorSantos, Oscar Fernando Pavão dos [UNIFESP]
dc.contributor.authorCanziani, Maria Eugênia Fernandes [UNIFESP]
dc.contributor.authorBoim, Mirian Aparecida [UNIFESP]
dc.contributor.authorGóes, Miguel Ângelo [UNIFESP]
dc.contributor.authorDraibe, Sergio Antonio [UNIFESP]
dc.contributor.authorBalakrishnan, V.
dc.contributor.authorCendoroglo Neto, Miguel [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionTufts Univ New England Med Ctr
dc.date.accessioned2018-06-15T17:44:16Z
dc.date.available2018-06-15T17:44:16Z
dc.date.issued2008-05-01
dc.description.abstractPurpose: Increased serum concentrations of soluble Fas (sFas) have been reported in patients with chronic kidney disease (CKD). However, little is known about the renal clearance of sFas, whether sFas is reabsorbed in the renal tubules, or the behavior of sFas synthesis in CKD.Materials and methods: We studied 69 patients with CKD (60 +/- 15 years old, creatinine clearance 37+19 ml/min/1.73 m(2)) and 14 healthy subjects (61 +/- 17 years, creatinine clearance 79 +/- 24 ml/min/1.73 m(2)). ELISA was used to measure the levels of sFas (pg/mL) and retinol binding protein (RBP - mg/L). RT-PCR was used to quantify sFasmRNA of leukocytes.Results: Serum sFas levels were significantly higher in patients with CKD (2781 +/- 1214 vs. 2196 +/- 773, p=0.02). The concentrations of sFas in 24-hour urine samples (23 +/- 27 vs. 40 +/- 17, p=0.006) and sFas Clearance (0.019 +/- 0.022 vs. 0.036 +/- 0.020, p=0.01) were significantly lower in patients with CKD. sFas clearance correlated with creatinine clearance (r=0.25, p=0.02). Urine concentrations of RBP correlated with sFas concentrations in the urine (r=0.80, p<0.001). sFasmRNA were higher in patients with CKD (3.9 +/- 1.8 vs. 2.5 +/- 0.9, p<0.001).Conclusions: In CKD patients, the decrease in renal function is followed by a decrease in sFas clearance and an increase in serum sFas. In patients with proximal tubule dysfunction (high urinary RBP concentrations), urinary sFas is also increased, suggesting that sFas is reabsorbed by the proximal tubule. It is possible that an increase in sFas synthesis also contributes to the increase of serum sFas concentrations in uremia.en
dc.description.affiliationUniv Fed Sao Paulo, Div Nephrol, UNIFESP, Dept Med, BR-04038002 Sao Paulo, Brazil
dc.description.affiliationTufts Univ New England Med Ctr, Dept Med, Tufts Sch Med, Div Nephrol, Boston, MA 02111 USA
dc.description.affiliationUnifespUniv Fed Sao Paulo, Div Nephrol, UNIFESP, Dept Med, BR-04038002 Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent405-410
dc.identifierhttp://www.artificial-organs.com/article/high-serum-levels-of-soluble-fas--sfas--in-ckd-patients--effects-of-renal-clearance--reabsorption-and-synthesis-art002304
dc.identifier.citationInternational Journal Of Artificial Organs. Milan: Wichtig Editore, v. 31, n. 5, p. 405-410, 2008.
dc.identifier.issn0391-3988
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/44020
dc.identifier.wosWOS:000257310700005
dc.language.isoeng
dc.publisherWichtig Editore
dc.relation.ispartofInternational Journal Of Artificial Organs
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectsFasen
dc.subjectRBPen
dc.subjectsFasmRNAen
dc.subjectchronic kidney diseasesen
dc.titleHigh serum levels of soluble Fas (sFas) in CKD patients: Effects of renal clearance, reabsorption and synthesisen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
EPM - Artigos