Novel strategy in Trypanosoma cruzi cell invasion: Implication of cholesterol and host cell microdomains

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dc.contributor.authorFernandes, Maria Cecilia
dc.contributor.authorCortez, Mauro
dc.contributor.authorGeraldo Yoneyama, Kelly Aparecida
dc.contributor.authorStraus, Anita Hilda
dc.contributor.authorYoshida, Nobuko
dc.contributor.authorMortara, Renato Arruda
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T13:49:11Z
dc.date.available2016-01-24T13:49:11Z
dc.date.issued2007-11-01
dc.description.abstractTrypanosoma cruzi, the etiological agent of Chagas' disease, is an obligatory intracellular parasite in the mammalian host. in order to invade a wide variety of mammalian cells, T cruzi engages parasite components that are differentially expressed among strains and infective forms. Because the identification of putative protein receptors has been particularly challenging, we investigated whether cholesterol and membrane rafts, sterol- and sphingolipid-enriched membrane domains, could be general host surface components involved in invasion of metacyclic trypomastigotes and extracellular amastigotes of two parasite strains with distinct infectivities. HeLa or Vero cells treated with methyl-beta-cyclodextrin (M beta CD) are less susceptible to invasion by both infective forms, and the effect was dose-dependent for trypomastigote but not amastigote invasion. Moreover, treatment of parasites with MPCD only inhibited trypomastigote invasion. Filipin labeling confirmed that host cell cholesterol concentrated at the invasion sites. Binding of a cholera toxin B subunit (CTX-B) to ganglioside GM1, a marker of membrane rafts, inhibited parasite infection. Cell labeling with CTX-B conjugated to fluorescein isothiocyanate revealed that not only cholesterol but also GM1 is implicated in parasite entry. These findings thus indicate that microdomains present in mammalian cell membranes, that are enriched in cholesterol and GM1, are involved in invasion by T cruzi infective forms. (c) 2007 Australian Society for Parasitology Inc. Published by Elsevier B.V. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Bioquim, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Bioquim, BR-04023062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent1431-1441
dc.identifierhttp://dx.doi.org/10.1016/j.ijpara.2007.04.025
dc.identifier.citationInternational Journal for Parasitology. Oxford: Pergamon-Elsevier B.V., v. 37, n. 13, p. 1431-1441, 2007.
dc.identifier.doi10.1016/j.ijpara.2007.04.025
dc.identifier.issn0020-7519
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/30126
dc.identifier.wosWOS:000250182800002
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofInternational Journal for Parasitology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectTrypanosoma cruzien
dc.subjecttrypomastigoteen
dc.subjectamastigoteen
dc.subjectmembrane raftsen
dc.subjectcholesterolen
dc.subjectcell invasionen
dc.titleNovel strategy in Trypanosoma cruzi cell invasion: Implication of cholesterol and host cell microdomainsen
dc.typeinfo:eu-repo/semantics/article
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