FURTHER CHARACTERIZATION OF THE ACIDIC GPI-HYDROLYZING PHOSPHOLIPASE PRESENT IN HUMAN SERA

dc.contributor.authorStambuk, Boris Juan Carlos Ugarte [UNIFESP]
dc.contributor.authorCardosodealmeida, M. L.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2018-06-15T14:28:06Z
dc.date.available2018-06-15T14:28:06Z
dc.date.issued1994-02-01
dc.description.abstractA phospholipase from human serum capable of hydrolyzing glycosylphosphatidylinositol membrane anchors was described and partially characterized by our group some years ago. This activity presented a pH optimum between 5.0 and 6.0 and was inhibited by EDTA, EGTA and 1,10-phenanthroline. Partial purification showed that the enzyme was a glycoprotein with an apparent molecular weight of 140 kDa as judged by gel filtration. Other investigators characterized at the same time a phospholipase D with similar properties but with a pH optimum near 7.5. We now confirm that the human serum enzyme is indeed a phospholipase D capable of hydrolyzing mfVSG and glycolipids A and C from T. brucei. Isoelectric focusing of whole sera suggests the presence of two isoforms, one with a pi of 4.7 which was the form previously purified by our group, and others with pI from 6.2 to 7.4.en
dc.description.affiliationESCOLA PAULISTA MED,DEPT MICROBIOL IMUNOL & PARASITOL,BR-04023062 SAO PAULO,SP,BRAZIL
dc.description.affiliationUnifespESCOLA PAULISTA MED,DEPT MICROBIOL IMUNOL & PARASITOL,BR-04023062 SAO PAULO,SP,BRAZIL
dc.description.provenanceMade available in DSpace on 2018-06-15T14:28:06Z (GMT). No. of bitstreams: 0 Previous issue date: 1994-02-01en
dc.description.sourceWeb of Science
dc.format.extent383-387
dc.identifier.citationBrazilian Journal Of Medical And Biological Research. Sao Paulo: Assoc Bras Divulg Cientifica, v. 27, n. 2, p. 383-387, 1994.
dc.identifier.issn0100-879X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/43052
dc.identifier.wosWOS:A1994MX60900040
dc.language.isoeng
dc.publisherAssoc Bras Divulg Cientifica
dc.relation.ispartofBrazilian Journal Of Medical And Biological Research
dc.rightsAcesso restrito
dc.subjectGPIen
dc.subjectPHOSPHOLIPASE Den
dc.subjectACIDIC LIPASEen
dc.titleFURTHER CHARACTERIZATION OF THE ACIDIC GPI-HYDROLYZING PHOSPHOLIPASE PRESENT IN HUMAN SERAen
dc.typeArtigo
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