Establishment and conventional cytogenetic characterization of three gastric cancer cell lines

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dc.contributor.authorLeal, Mariana Ferreira [UNIFESP]
dc.contributor.authorMartins do Nascimento, Jose Luiz
dc.contributor.authorAraujo da Silva, Carla Elvira
dc.contributor.authorVita Lamarao, Maria Fernanda
dc.contributor.authorCalcagno, Danielle Queiroz
dc.contributor.authorKhayat, Andre Salim [UNIFESP]
dc.contributor.authorAssumpcao, Paulo Pimentel
dc.contributor.authorCabral, Isabel Rosa
dc.contributor.authorCardoso Smith, Marilia de Arruda [UNIFESP]
dc.contributor.authorBurbano, Rommel Rodriguez
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionFed Univ Para
dc.date.accessioned2016-01-24T13:58:53Z
dc.date.available2016-01-24T13:58:53Z
dc.date.issued2009-11-01
dc.description.abstractGastric cancer is the fourth most frequent type of cancer and the second most frequent cause of cancer mortality worldwide. Only a modest number of gastric carcinoma cell lines have been isolated thus far. Here we describe the establishment and cytogenetic characterization of three new gastric cancer cell lines obtained from primary gastric adenocarcinoma (ACP02 and ACP03) and cancerous ascitic fluid (AGP01) of individuals from northern Brazil. ACP02, ACP03, and AGP01 cell lines are presently in the 60th passage. the cell lines grew in a disorganized single layer with some agglomerations and heterogeneous divisions (bipolar and multipolar). All cell lines exhibited a composite karyotype with several clonal chromosome alterations. Trisomy 8 was the most frequent alteration. Chromosome 8 aneusomy was confirmed by fluorescence in situ hybridization. All cell lines also exhibited trisomy 7 and deletion of chromosome arm 17p. These results suggest that, although frequent chromosome alterations are commonly observed due to culture process, the ACP02, ACP03, and AGP01 cell lines and primary gastric cancer from individuals of northern Brazil share genetic alterations, supporting use of these cell lines as a model of gastric carcinogenesis in this population. (C) 2009 Elsevier Inc. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Morphol & Genet, Div Genet, BR-04023900 São Paulo, Brazil
dc.description.affiliationFed Univ Para, Inst Biol Sci, Lab Cellular & Mol Neurochem, BR-66059 Belem, Para, Brazil
dc.description.affiliationFed Univ Para, Inst Biol Sci, Human Cytogenet Lab, BR-66059 Belem, Para, Brazil
dc.description.affiliationFed Univ Para, Joao Barros Barreto Univ Hosp, BR-66059 Belem, Para, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Morphol & Genet, Div Genet, BR-04023900 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipNational Council for Scientific and Technological Development
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent85-91
dc.identifierhttp://dx.doi.org/10.1016/j.cancergencyto.2009.04.020
dc.identifier.citationCancer Genetics and Cytogenetics. New York: Elsevier B.V., v. 195, n. 1, p. 85-91, 2009.
dc.identifier.doi10.1016/j.cancergencyto.2009.04.020
dc.identifier.issn0165-4608
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/31923
dc.identifier.wosWOS:000271562400015
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofCancer Genetics and Cytogenetics
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.titleEstablishment and conventional cytogenetic characterization of three gastric cancer cell linesen
dc.typeinfo:eu-repo/semantics/article
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