Microbiopsies of Surface Dental Enamel as a Tool to Measure Body Lead Burden

dc.contributor.authorOlympio, Kelly Polido Kaneshiro
dc.contributor.authorNaozuka, Juliana
dc.contributor.authorMagalhaes, Ana Carolina
dc.contributor.authorPera Garcia, Manuel Valentim de
dc.contributor.authorOliveira, Pedro Vitoriano de
dc.contributor.authorRabelo Buzalaf, Marilia Afonso
dc.contributor.authorBechara, Etelvino José Henriques [UNIFESP]
dc.contributor.authorGüenther, Wanda Maria Risso
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T13:59:08Z
dc.date.available2016-01-24T13:59:08Z
dc.date.issued2010-01-01
dc.description.abstractLead (Pb) poisoning is preventable but continues to be a public health problem in several countries. Measuring Pb in the surface dental enamel (SDE) using microbiopsies is a rapid, safe, and painless procedure. There are different protocols to perform these microbiopsies, but the reliability of dental enamel lead levels (DELL) determination is dependent upon biopsy depth (BD). It is established that DELL decrease from the outermost superficial layer to the inner layer of dental enamel. the aim of this study was to determine DELL obtained by two different microbiopsy techniques on SDE termed protocol I and protocol II. Two consecutive enamel layers were removed from the same subject group (n = 138) for both protocols. Protocol I consisted of a biopsied site with a diameter of 4 mm after the application of 10 l HCl for 35 s. Protocol II involved a biopsied site of 1.6 mm diameter after application of 5 l HCl for 20 s. the results demonstrated that there were no significant differences for BD and DELL between homologous teeth using protocol I. However, there was a significant difference between DELL in the first and second layers using both protocols. Further, the BD in protocol II overestimated DELL values. in conclusion, SDE analyzed by microbiopsy is a reliable biomarker in protocol I, but the chemical method to calculate BD in protocol II appeared to be inadequate for measurement of DELL. Thus, DELL could not be compared among studies that used different methodologies for SDE microbiopsies.en
dc.description.affiliationUniv São Paulo, Fac Saude Publ, Dept Saude Ambiental, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Inst Quim, Dept Quim Analit, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Dept Ciencias Biol, Fac Odontol Bauru, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Dept Matemat Aplicada, Inst Matemat & Estat, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Inst Quim, Dept Bioquim, BR-01498 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Ciencias Exatas & Terra, Diadema, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Ciencias Exatas & Terra, Diadema, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipProjeto Milenio Redoxoma
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIDFAPESP: 01/09641-1
dc.description.sponsorshipIDFAPESP: 06/56530-4
dc.format.extent627-636
dc.identifierhttp://dx.doi.org/10.1080/15287390903578224
dc.identifier.citationJournal of Toxicology and Environmental Health-part A-current Issues. Philadelphia: Taylor & Francis Inc, v. 73, n. 9, p. 627-636, 2010.
dc.identifier.doi10.1080/15287390903578224
dc.identifier.issn1528-7394
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/32133
dc.identifier.wosWOS:000277566600007
dc.language.isoeng
dc.publisherTaylor & Francis Inc
dc.relation.ispartofJournal of Toxicology and Environmental Health-part A-current Issues
dc.rightsAcesso restrito
dc.rights.licensehttp://journalauthors.tandf.co.uk/permissions/reusingOwnWork.asp
dc.titleMicrobiopsies of Surface Dental Enamel as a Tool to Measure Body Lead Burdenen
dc.typeArtigo
Arquivos
Coleções