Effects of lidocaine with epinephrine or with buffer on wound healing in rat skin

dc.contributor.authorRodrigues, Felipe V. [UNIFESP]
dc.contributor.authorHochman, Bernardo [UNIFESP]
dc.contributor.authorWood, Viviane T. [UNIFESP]
dc.contributor.authorSimoes, Manuel J. [UNIFESP]
dc.contributor.authorJuliano, Yara [UNIFESP]
dc.contributor.authorFerreira, Lydia M. [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:06:18Z
dc.date.available2016-01-24T14:06:18Z
dc.date.issued2011-03-01
dc.description.abstractLidocaine blocks nociceptive fibers, preventing initial wound signaling and mast cell degranulation. It is hypothesized that epinephrine and buffer affect the wound healing by potentiating lidocaine blockage. This double-blind, randomized, self-controlled study investigated this possibility using male Wistar rats, which were injected with 2 mL of different solutions into the left and right sides of the back. Then, each side was incised and sutured. Sixty rats were divided in three groups: saline solution (SS) and lidocaine; lidocaine and lidocaine with buffer; lidocaine with epinephrine and lidocaine with epinephrine and buffer. Half of each group was sacrificed 7 days after surgery and the remaining after 21 days. A sample of each wound was obtained and quantified for the level of collagen present using computer morphometry and for mast cell quantity. There were no differences between animals with regard to the collagen. However, mast cell levels in the same animal significantly differed between SS x lidocaine. Comparison of the same injected substance between animals with different healing dates showed a significant effect on collagen SS and on all mast cells, except SS. Lidocaine affected collagenization and decreased the initial quantity of mast cells at the wound site.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Plast & Reconstruct Surg, Hosp São Paulo, Paulista Med Sch, BR-04024900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Histol & Struct Biol, Paulista Med Sch, BR-04024900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Epidemiol, Paulista Med Sch, BR-04024900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Plast & Reconstruct Surg, Hosp São Paulo, Paulista Med Sch, BR-04024900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Histol & Struct Biol, Paulista Med Sch, BR-04024900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Epidemiol, Paulista Med Sch, BR-04024900 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao e Amparo a Pesquisa de São Paulo
dc.description.sponsorshipDepartamento de Cirurgia Plastica da Universidade Federal de São Paulo
dc.format.extent223-228
dc.identifierhttp://dx.doi.org/10.1111/j.1524-475X.2010.00654.x
dc.identifier.citationWound Repair and Regeneration. Malden: Wiley-Blackwell, v. 19, n. 2, p. 223-228, 2011.
dc.identifier.doi10.1111/j.1524-475X.2010.00654.x
dc.identifier.issn1067-1927
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/33545
dc.identifier.wosWOS:000287878100232
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofWound Repair and Regeneration
dc.rightsAcesso restrito
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.titleEffects of lidocaine with epinephrine or with buffer on wound healing in rat skinen
dc.typeArtigo
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