An evaluation of chondrocyte morphology and gene expression on superhydrophilic vertically-aligned multi-walled carbon nanotube films

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dc.contributor.authorAntonioli, Eliane
dc.contributor.authorLobo, Anderson O.
dc.contributor.authorFerretti, Mario [UNIFESP]
dc.contributor.authorCohen, Moises [UNIFESP]
dc.contributor.authorMarciano, Fernanda R.
dc.contributor.authorCorat, Evaldo J.
dc.contributor.authorTrava-Airoldi, Vladimir J.
dc.contributor.institutionHosp Israelita Albert Einstein
dc.contributor.institutionUniv Vale Paraiba
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionInst Nacl Pesquisas Espaciais
dc.date.accessioned2016-01-24T14:31:20Z
dc.date.available2016-01-24T14:31:20Z
dc.date.issued2013-03-01
dc.description.abstractCartilage serves as a low-friction and wear-resistant articulating surface in diarthrodial joints and is also important during early stages of bone remodeling. Recently, regenerative cartilage research has focused on combinations of cells paired with scaffolds. Superhydrophilic vertically aligned carbon nanotubes (VACNTs) are of particular interest in regenerative medicine. the aim of this study is to evaluate cell expansion of human articular chondrocytes on superhydrophilic VACNTs, as well as their morphology and gene expression. VACNT films were produced using a microwave plasma chamber on Ti substrates and submitted to an O-2 plasma treatment to make them superhydrophilic. Human chondrocytes were cultivated on superhydrophilic VACNTs up to five days. Quantitative RT-PCR was performed to measure type I and type II Collagen, Sox9, and Aggrecan mRNA expression levels. the morphology was analyzed by scanning electron microscopy (SEM) and confocal microscopy. SEM images demonstrated that superhydrophilic VACNTs permit cell growth and adhesion of human chondrocytes. the chondrocytes had an elongated morphology with some prolongations. Chondrocytes cultivated on superhydrophilic VACNTs maintain the level expression of Aggrecan, Sox9, and Collagen II determined by gPCR. This study was the first to indicate that superhydrophilic VACNTs may be used as an efficient scaffold for cartilage or bone repair. (C) 2012 Elsevier B.V. All rights reserved.en
dc.description.affiliationHosp Israelita Albert Einstein, Res & Educ Inst, São Paulo, Brazil
dc.description.affiliationUniv Vale Paraiba, Lab Biomed Nanotechnol, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Ortophed Div, São Paulo, Brazil
dc.description.affiliationInst Nacl Pesquisas Espaciais, Lab Associado Sensores & Mat, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Ortophed Div, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: Proc: 2011/17877-7
dc.description.sponsorshipIDFAPESP: 2011/20345-7
dc.format.extent641-647
dc.identifierhttp://dx.doi.org/10.1016/j.msec.2012.10.010
dc.identifier.citationMaterials Science & Engineering C-materials for Biological Applications. Amsterdam: Elsevier B.V., v. 33, n. 2, p. 641-647, 2013.
dc.identifier.doi10.1016/j.msec.2012.10.010
dc.identifier.issn0928-4931
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/36037
dc.identifier.wosWOS:000315761700011
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofMaterials Science & Engineering C-materials for Biological Applications
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectMultiwalled carbon nanotubesen
dc.subjectVertically aligneden
dc.subjectSuperhydrophilicen
dc.subjectEndochondral ossificationen
dc.subjectChondrocytesen
dc.subjectGene expressionen
dc.titleAn evaluation of chondrocyte morphology and gene expression on superhydrophilic vertically-aligned multi-walled carbon nanotube filmsen
dc.typeinfo:eu-repo/semantics/article
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