Effects of indocyanine green injection on the retinal surface and into the subretinal space in rabbits

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dc.contributor.authorMaia, M.
dc.contributor.authorKellner, L.
dc.contributor.authorDe Juan, E.
dc.contributor.authorSmith, R.
dc.contributor.authorFarah, M. E.
dc.contributor.authorMargalit, E.
dc.contributor.authorLakhanpal, R. R.
dc.contributor.authorGrebe, L.
dc.contributor.authorEong, KGA
dc.contributor.authorHumayun, M. S.
dc.contributor.institutionUniv So Calif
dc.contributor.institutionJohns Hopkins Univ
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:34:17Z
dc.date.available2016-01-24T12:34:17Z
dc.date.issued2004-02-01
dc.description.abstractPurpose: To evaluate the effects of indocyanine green (ICG) injection on the retinal surface and into the subretinal space of rabbit eyes.Methods: Twenty-two Dutch-belted rabbits underwent two-port vitrectomy followed by injection of ICG (5 mg/mL) on the retinal surface and into the subretinal space. Balanced salt solution (BSS) was also injected subretinally. the locations where ICG was delivered (both epiretinal and subretinal) were exposed to light from an endoilluminator for 7 minutes. the animals were examined at 1, 7, and 14 days after surgery. the eyes were studied by fluorescein angiography as well as light and electron microscopy.Results: No damage was observed after epiretinal ICG injection, but subretinal ICG injection resulted in damage to the outer nuclear layer, photoreceptor inner and outer segments, and retinal pigment epithelium. This damage was more severe with longer follow-up. Control experiments without ICG, in which balanced salt solution was injected into the subretinal space or light was delivered on the epiretinal surface, demonstrated only damage to the photoreceptor outer segments.Conclusion: Subretinal delivery of ICG (5 mg/mL) in rabbits induces retinal pigment epithelium, photoreceptor inner and outer segment, and outer nuclear layer damage. These mechanisms of damage may explain the retinal pigment epithelium changes that are sometimes seen after ICG-assisted internal limiting membrane peeling in humans.en
dc.description.affiliationUniv So Calif, Keck Sch Med, Doheny Eye Inst, Doheny Retina Inst, Los Angeles, CA 90033 USA
dc.description.affiliationJohns Hopkins Univ, Sch Med, Wilmer Ophthalmol Inst, Baltimore, MD 21218 USA
dc.description.affiliationUniversidade Federal de São Paulo, Paulista Sch Med, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Paulista Sch Med, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent80-91
dc.identifierhttp://dx.doi.org/10.1097/00006982-200402000-00012
dc.identifier.citationRetina-the Journal of Retinal and Vitreous Diseases. Philadelphia: Lippincott Williams & Wilkins, v. 24, n. 1, p. 80-91, 2004.
dc.identifier.doi10.1097/00006982-200402000-00012
dc.identifier.issn0275-004X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/27623
dc.identifier.wosWOS:000189234200012
dc.language.isoeng
dc.publisherLippincott Williams & Wilkins
dc.relation.ispartofRetina-the Journal of Retinal and Vitreous Diseases
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.titleEffects of indocyanine green injection on the retinal surface and into the subretinal space in rabbitsen
dc.typeinfo:eu-repo/semantics/article
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