The fate of plasma kallikrein in normal and kininogen-deficient rats

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dc.contributor.authorKouyoumdjian, M.
dc.contributor.authorDamas, J.
dc.contributor.institutionUniv Liege
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:30:32Z
dc.date.available2016-01-24T12:30:32Z
dc.date.issued1998-02-01
dc.description.abstractThe clearance of exogenous plasma kallikrein, its uptake by liver, spleen, kidneys, lungs and its extravasation in the paws were determined in normal Wistar rats, normal and kininogen-deficient Brown Norway rats. Kallikrein was purified from rat plasma and labelled with I-125. After intravenous injection of I-125-kallikrein, the disappearance of acid-precipitable kallikrein from the blood fits a biexponential curve similar in the three groups of rats: a rapid initial clearance (T-1/2 around 3 min) followed by a phase of slower elimination (T-1/2 around 50 min). Removal of kallikrein from the blood was associated with a large uptake of radioactivity by the liver: 67% of the I-125-kallikrein cleared from the blood at 10 min. the kidneys and the spleen accumulated small amounts of the radioactivity. the uptake of kallikrein by the spleen was slightly reduced in kininogen-deficient rats. the kininogen deficiency in Brown Norway rats from the strain BN/May Pfd was confirmed by the low levels of kinins released by tissue kallikrein and by a prolongation of activated thromboplastin times in the plasma of these animals. We concluded that plasma kallikrein is rapidly cleared from the circulation of the rat. the liver is the main clearing organ of plasma kallikrein. the disappearance of kallikrein from the circulation is not affected by the lack of high molecular weight kininogen, except in the case of the uptake of the enzyme performed by the cells of the spleen, which is reduced.en
dc.description.affiliationUniv Liege, Inst Leon Fredericq, Fac Med, B-4020 Liege, Belgium
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Biochem, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Biochem, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent25-32
dc.identifierhttp://dx.doi.org/10.1076/apab.106.1.25.4400
dc.identifier.citationArchives of Physiology and Biochemistry. Lisse: Swets Zeitlinger Publishers, v. 106, n. 1, p. 25-32, 1998.
dc.identifier.doi10.1076/apab.106.1.25.4400
dc.identifier.issn1381-3455
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/25862
dc.identifier.wosWOS:000076117000005
dc.language.isoeng
dc.publisherSwets Zeitlinger Publishers
dc.relation.ispartofArchives of Physiology and Biochemistry
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectclearanceen
dc.subjectkallikreinen
dc.subjectkininogenen
dc.subjectraten
dc.titleThe fate of plasma kallikrein in normal and kininogen-deficient ratsen
dc.typeinfo:eu-repo/semantics/article
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