Effect of epithelial debridement on human cornea proteoglycans

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dc.contributor.authorSoriano, Eduardo Sone [UNIFESP]
dc.contributor.authorCampos, Mauro Silveira de Queiroz [UNIFESP]
dc.contributor.authorAguiar, Jair Adriano Kopke [UNIFESP]
dc.contributor.authorMichelacci, Yara Maria [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2015-06-14T13:29:20Z
dc.date.available2015-06-14T13:29:20Z
dc.date.issued2001-03-01
dc.description.abstractCorneal transparency is attributed to the regular spacing and diameter of collagen fibrils, and proteoglycans may play a role in fibrillogenesis and matrix assembly. Corneal scar tissue is opaque and this opacity is explained by decreased ultrastructural order that may be related to proteoglycan composition. Thus, the objectives of the present study were to characterize the proteoglycans synthesized by human corneal explants and to investigate the effect of mechanical epithelial debridement. Human corneas unsuitable for transplants were immersed in F-12 culture medium and maintained under tissue culture conditions. The proteoglycans synthesized in 24 h were labeled metabolically by the addition of 35S-sulfate to the medium. These compounds were extracted by 4 M GuHCl and identified by a combination of agarose gel electrophoresis, enzymatic degradation with protease and mucopolysaccharidases, and immunoblotting. Decorin was identified as the main dermatan sulfate proteoglycan and keratan sulfate proteoglycans were also prominent components. When the glycosaminoglycan side chains were analyzed, only keratan sulfate and dermatan sulfate were detected (~50% each). Nevertheless, when these compounds were 35S-labeled metabolically, the label in dermatan sulfate was greater than in keratan sulfate, suggesting a lower synthesis rate for keratan sulfate. 35S-Heparan sulfate also appeared. The removal of the epithelial layer caused a decrease in heparan sulfate labeling and induced the synthesis of dermatan sulfate by the stroma. The increased deposit of dermatan sulfate proteoglycans in the stroma suggests a functional relationship between epithelium and stroma that could be related to the corneal opacity that may appear after epithelial cell debridement.en
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de Bioquímica
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de Oftalmologia
dc.description.affiliationUnifespUNIFESP, EPM, Depto. de Bioquímica
dc.description.affiliationUnifespUNIFESP, EPM, Depto. de Oftalmologia
dc.description.sourceSciELO
dc.format.extent325-331
dc.identifierhttp://dx.doi.org/10.1590/S0100-879X2001000300005
dc.identifier.citationBrazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 34, n. 3, p. 325-331, 2001.
dc.identifier.doi10.1590/S0100-879X2001000300005
dc.identifier.fileS0100-879X2001000300005.pdf
dc.identifier.issn0100-879X
dc.identifier.scieloS0100-879X2001000300005
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/1128
dc.identifier.wosWOS:000167640800005
dc.language.isoeng
dc.publisherAssociação Brasileira de Divulgação Científica
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectcorneal explantsen
dc.subjectde-epithelizationen
dc.subjectglycosaminoglycanen
dc.subjectproteoglycanen
dc.titleEffect of epithelial debridement on human cornea proteoglycansen
dc.typeinfo:eu-repo/semantics/article
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