Antithrombin stabilisation by sulfated carbohydrates correlates with anticoagulant activity

dc.contributor.authorLima, Marcelo A. [UNIFESP]
dc.contributor.authorHughes, Ashley J.
dc.contributor.authorVeraldi, Noemi
dc.contributor.authorRudd, Timothy R.
dc.contributor.authorHussain, Rohanah
dc.contributor.authorBrito, Adriana S. [UNIFESP]
dc.contributor.authorChavante, Suely F.
dc.contributor.authorTersariol, Ivarne I. [UNIFESP]
dc.contributor.authorSiligardi, Giuliano
dc.contributor.authorNader, Helena B. [UNIFESP]
dc.contributor.authorYates, Edwin A.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionDiamond Light Source Ltd
dc.contributor.institutionUniv Liverpool
dc.contributor.institutionIst Ric Chim & Biochim G Ronzoni
dc.contributor.institutionUniv Fed Rio Grande do Norte
dc.description.abstractThermal stabilisation of native antithrombin-III (AT), determined using differential scanning fluorimetry, correlated with the anticoagulant activity of heparin and heparin-related saccharides. Similar conformational changes were induced in native AT by a variety of active and inactive heparin-related sulfated carbohydrates, measured in solution using synchrotron radiation circular dichroism, and their anticoagulant activities. Measurement of native AT stabilisation provides a convenient assay for prospective anticoagulants and represents an additional parameter by which to compare biosimilar heparins.en
dc.description.affiliationUniversidade Federal de São Paulo UNIFESP, Dept Biochem, BR-04044020 São Paulo, Brazil
dc.description.affiliationDiamond Light Source Ltd, Beam Line Circular Dichroism 23, Didcot OX11 ODE, Oxon, England
dc.description.affiliationUniv Liverpool, Dept Struct & Chem Biol, Liverpool L69 7ZB, Merseyside, England
dc.description.affiliationIst Ric Chim & Biochim G Ronzoni, I-20133 Milan, Italy
dc.description.affiliationUniv Fed Rio Grande do Norte, Dept Biochem, BR-59072970 Natal, RN, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo UNIFESP, Dept Biochem, BR-04044020 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipNational Science Foundation's National High Magnetic Field Laboratory User Program in the Advanced Magnetic Resonance Imaging and Spectroscopy (AMRIS) Facility (McKnight Brain Institute, University of Florida, USA)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.identifier.citationMedchemcomm. Cambridge: Royal Soc Chemistry, v. 4, n. 5, p. 870-873, 2013.
dc.publisherRoyal Soc Chemistry
dc.rightsAcesso restrito
dc.titleAntithrombin stabilisation by sulfated carbohydrates correlates with anticoagulant activityen