Distribution of epitopes of Trypanosoma cruzi amastigotes during the intracellular life cycle within mammalian cells

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dc.contributor.authorBarros, H. C.
dc.contributor.authorVerbisck, N. V.
dc.contributor.authorDaSilva, S.
dc.contributor.authorAraguth, M. F.
dc.contributor.authorMortara, R. A.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:30:23Z
dc.date.available2016-01-24T12:30:23Z
dc.date.issued1997-07-01
dc.description.abstractIn this study we have examined the distribution of epitopes defined by monoclonal antibodies raised against Trypanosoma cruzi amastigotes during the intracellular life cycle of the parasite. We have raised monoclonal antibodies towards amastigote forms and performed preliminary immunochemical characterization of their reactivities. MAB 1D9, 3G8, 2B7, 3B9, and 4B9 react with carbohydrate epitopes of the parasite major surface glycoprotein-Ssp-4 defined by MAB 2C2 [5]; MAB 4B5 reacts with a noncarbohydrate epitope in all developmental stages of the parasite, and MAB 3B2 also detects a noncarbohydrate epitope preferentially in T. cruzi flagellated forms. Vero cells infected with tissue culture-derived trypomastigotes of clone D11 (G strain) were fixed at different times during the intracellular proliferation of parasites, and processed for immune-electron microscopy and confocal immunofluorescence with the different monoclonal antibodies. We observed that while the surface distribution of MAB 2C2 and 4B9 epitopes was uniform throughout the cycle, MAB 1D9, 3G8, and 2B7 reacted with cytoplasmic membrane-bound compartments of the amastigotes. MAB 3B9 displayed a unique surface dentate pattern in some amastigotes. MAB 4B5 recognized a curved-shaped structure at the flagellar pocket region in some intracellular amastigotes and localized to the membrane in dividing forms. in intracellular trypomastigotes, MAB 4B5 also displayed a punctate pattern near the flagellar pocket.en
dc.description.affiliationUniversidade Federal de São Paulo,DEPT MICROBIOL IMMUNOL & PARASITOL,BR-04023062 São Paulo,BRAZIL
dc.description.affiliationUniversidade Federal de São Paulo,CTR MICROSCOPIA ELETRON,ESCOLA PAULISTA MED,BR-04023062 São Paulo,BRAZIL
dc.description.affiliationUnifespUniversidade Federal de São Paulo,DEPT MICROBIOL IMMUNOL & PARASITOL,BR-04023062 São Paulo,BRAZIL
dc.description.affiliationUnifespUniversidade Federal de São Paulo,CTR MICROSCOPIA ELETRON,ESCOLA PAULISTA MED,BR-04023062 São Paulo,BRAZIL
dc.description.sourceWeb of Science
dc.format.extent332-344
dc.identifierhttp://dx.doi.org/10.1111/j.1550-7408.1997.tb05675.x
dc.identifier.citationJournal of Eukaryotic Microbiology. Lawrence: Soc Protozoologists, v. 44, n. 4, p. 332-344, 1997.
dc.identifier.doi10.1111/j.1550-7408.1997.tb05675.x
dc.identifier.issn1066-5234
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/25761
dc.identifier.wosWOS:A1997XJ82800009
dc.language.isoeng
dc.publisherSoc Protozoologists
dc.relation.ispartofJournal of Eukaryotic Microbiology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectamastigote antigensen
dc.subjectconfocal microscopyen
dc.subjectimmuno-electron microscopyen
dc.subjectmonoclonal antibodiesen
dc.titleDistribution of epitopes of Trypanosoma cruzi amastigotes during the intracellular life cycle within mammalian cellsen
dc.typeinfo:eu-repo/semantics/article
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