Differences in neonatal exposure to estradiol or testosterone on ovarian function and hormonal levels

dc.contributor.authorMarcondes, Rodrigo R.
dc.contributor.authorCarvalho, Katia C.
dc.contributor.authorDuarte, Daniele C.
dc.contributor.authorGarcia, Natalia
dc.contributor.authorAmaral, Vinicius C.
dc.contributor.authorSimoes, Manuel J. [UNIFESP]
dc.contributor.authorLo Turco, Edson G. [UNIFESP]
dc.contributor.authorSoares, Jose M.
dc.contributor.authorBaracat, Edmund C.
dc.contributor.authorMaciel, Gustavo A. R.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:40:00Z
dc.date.available2016-01-24T14:40:00Z
dc.date.issued2015-02-01
dc.description.abstractExposure to an excess of androgen or estrogen can induce changes in reproductive function in adult animals that resemble polycystic ovary syndrome in humans. However, considerable differences exist among several types of animal models. Little is known about the molecular features of steroidogenesis and folliculogenesis in the ovaries of rats exposed to different sex steroids as neonates. Here, we evaluated the impact of androgen and estrogen exposure on the ovaries of adult female rats during their neonatal period in the gene expression of Lhr and Cyp17a1, two key players of steroidogenesis. We also assessed hormone levels, folliculogenesis and the theca-interstitial cell population. the study was performed on the second postnatal day in thirty female Wistar rats that were sorted into the following three intervention groups: testosterone, estradiol and vehicle (control group). the animals were euthanized 90 days after birth. the main outcomes were hormone serum levels, ovary histomorphometry and gene expression of Lhr and Cyp17a1 as analyzed via quantitative real-time PCR. We found that exposure to excess testosterone in early life increased the LH and testosterone serum levels, the LH/FSH ratio, ovarian theca-interstitial area and gene expression of Lhr and Cyp17a1 in adult rats. Estrogen induced an increase in the ovarian theca-interstitial area, the secondary follicle population and gene expression of Lhr and Cyp17a1. All animals exposed to the sex steroids presented with closed vaginas. Our data suggest that testosterone resulted in more pronounced reproductive changes than did estrogen exposure. Our results might provide some insight into the role of different hormones on reproductive development and on the heterogeneity of clinical manifestations of conditions such as polycystic ovary syndrome. (C) 2015 Elsevier Inc. All rights reserved.en
dc.description.affiliationUniv São Paulo, Fac Med, Disciplina Ginecol, Lab Ginecol Estrutural & Mol LIM 58, BR-01246903 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Morfol & Genet, Disciplina Histol & Biol Estrutural, BR-04023900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Cirurgia, Disciplina Urol, Setor Reprod Humana, BR-04024002 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Morfol & Genet, Disciplina Histol & Biol Estrutural, BR-04023900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Cirurgia, Disciplina Urol, Setor Reprod Humana, BR-04024002 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIDFAPESP: 2010/17417-3
dc.description.sponsorshipIDCNPq: 134694/2010-4
dc.format.extent28-33
dc.identifierhttp://dx.doi.org/10.1016/j.ygcen.2015.01.006
dc.identifier.citationGeneral and Comparative Endocrinology. San Diego: Academic Press Inc Elsevier Science, v. 212, p. 28-33, 2015.
dc.identifier.doi10.1016/j.ygcen.2015.01.006
dc.identifier.issn0016-6480
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/38708
dc.identifier.wosWOS:000352176000004
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofGeneral and Comparative Endocrinology
dc.rightsAcesso restrito
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectPolycystic ovary syndromeen
dc.subjectAnimal modelsen
dc.subjectLH receptoren
dc.subjectSteroiden
dc.subject17-Alpha-hydroxylaseen
dc.titleDifferences in neonatal exposure to estradiol or testosterone on ovarian function and hormonal levelsen
dc.typeArtigo
Arquivos
Coleções