Random amplified polymorphic DNA profiles as a tool for the characterization of Brazilian keratitis isolates of the genus Acanthamoeba

Random amplified polymorphic DNA profiles as a tool for the characterization of Brazilian keratitis isolates of the genus Acanthamoeba

Author Alves, J.m.p. Google Scholar
Gusmão, C.x. Google Scholar
Teixeira, M.m.g. Google Scholar
Freitas, Denise de Autor UNIFESP Google Scholar
Foronda, A.s. Google Scholar
Affonso, H.t. Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract The genus Acanthamoeba comprises free-living amebae identified as opportunistic pathogens of humans and other animal species. Morphological, biochemical and molecular approaches have shown wide genetic diversity within the genus. In an attempt to determine the genetic relatedness among isolates of Acanthamoeba we analyzed randomly amplified polymorphic DNA (RAPD) profiles of 11 Brazilian isolates from cases of human keratitis and 8 American type culture collection (ATCC) reference strains. We found that ATCC strains belonging to the same species present polymorphic RAPD profiles whereas strains of different species show very similar profiles. Although most Brazilian isolates could not be assigned with certainty to any of the reference species, they could be clustered according to pattern similarities. The results show that RAPD analysis is a useful tool for the rapid characterization of new isolates and the assessment of genetic relatedness of Acanthamoeba spp. A comparison between RAPD analyses and morphological characteristics of cyst stages is also discussed.
Keywords Acanthamoeba
keratitis
RAPD typing
genetic variation
Language English
Date 2000-01-01
Published in Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 33, n. 1, p. 19-26, 2000.
ISSN 0100-879X (Sherpa/Romeo, impact factor)
Publisher Associação Brasileira de Divulgação Científica
Extent 19-26
Origin http://dx.doi.org/10.1590/S0100-879X2000000100003
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000085031800003
SciELO ID S0100-879X2000000100003 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/880

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