Comparative efficacy of Belzer or Euro-Collins solutions for pancreatic preservation during cold ischemic storage in rats

Comparative efficacy of Belzer or Euro-Collins solutions for pancreatic preservation during cold ischemic storage in rats

Author Perez, Rogerio Renato Google Scholar
Goldenberg, Alberto Autor UNIFESP Google Scholar
Netto, Alcides Augusto Salzedas Google Scholar
Gonzalez, Adriano Miziara Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract PURPOSE:To compare the efficacy of different types of solutions (Belzer or Euro-Collins) for the preservation of rat pancreas during cold ischemia. METHODS:Thirty Wistar rats were divided into three groups according to the perfusion or storage solution: Group E (perfusion and storage in Euro-Collins solution); Group B (perfusion and storage in Belzer solution) and Group BE (Perfusion in Belzer solution and storage in Euro-Collins solution). After perfusion, the pancreas was excised and stored at 4˚C for 18 hours. Amylase was measured at 6, 12 and 18h, and histological analysis of the pancreas was performed after 18h of cold storage.RESULTS:Amylase was elevated and comparable in Groups E and BE after 12 and 18 hours of ischemia (p<0.05). In the exocrine pancreas, histological differences in the amount of necrosis (p=0.049), lymphocytic infiltrate (p<0.001) and neutrophilic infiltrate (p=0.004) were observed, with more favorable features present in Group B. In the endocrine pancreas, Group B showed less edema (p<0.001), but other parameters were similar among all groups. CONCLUSION:The Euro-Collins solution is inferior to the Belzer solution for the preservation of rat pancreas during cold ischemia.
Keywords Diabetes Mellitus, Type 1
Islets of Langerhans Transplantation
Organ Preservation Solutions
Rats
Language English
Date 2014-03-01
Published in Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, n. 3, p. 171-177, 2014.
ISSN 0102-8650 (Sherpa/Romeo, impact factor)
Publisher Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Extent 171-177
Origin http://dx.doi.org/10.1590/S0102-86502014000300005
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000333047100005
SciELO ID S0102-86502014000300171 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/8286

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