The effect of n-acetylcysteine on hepatic histomorphology during hypothermic preservation

The effect of n-acetylcysteine on hepatic histomorphology during hypothermic preservation

Author Risso, Patricia Sayuri Google Scholar
Koike, Marcia Kiyomi Google Scholar
Abrahão, Marcos de Souza Autor UNIFESP Google Scholar
Ferreira, Natalie Chaves Google Scholar
Montero, Edna Frasson de Souza Autor UNIFESP Google Scholar
Institution University City of São Paulo
São Paulo University Medical School
Universidade Federal de São Paulo (UNIFESP)
Abstract PURPOSE:To evaluate the NAC effects on liver hypothermic preservation at different time intervals.METHODS:For this, we used livers of male Wistar rats weighing between 250 and 300g, undergoing preservation in Ringer solution at 4°C for up to 24 hours. Tissue samples were obtained at four moments of preservation for histological examination by hematoxylin and eosin staining: T0 = beginning of preservation, T12 = 12 hours, T18 = 18 hours and T24 = 24 hours. Will be analyzed vacuolation, hepatic apoptosis by optical microscopy and parenchymal.RESULTS: The results showed a progressive increase in hepatic injury in both groups and showed that NAC was effective at T0. The parenchyma preservation was better in the NAC group and no difference when vacuolization of the cells.CONCLUSION: Hypothermic preservation, over time, causes changes in the hepatic parenchyma with increased apoptosis, loss of architecture, vacuolization, culminating in severe injury. The administration of N-acetylcysteine protects against preservation liver injury.
Keywords Acetylcysteine
Liver
Hypothermia
Organ Preservation
Rats
Language English
Date 2014-01-01
Published in Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, p. 28-32, 2014.
ISSN 0102-8650 (Sherpa/Romeo, impact factor)
Publisher Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Extent 28-32
Origin http://dx.doi.org/10.1590/S0102-86502014001700006
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000344466300007
SciELO ID S0102-86502014001500028 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/8193

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