A manual method to obtain platelet rich plasma
Marques, Fabiana Paulino
Ingham, Sheila Jean McNeill [UNIFESP]
Franciozi, Carlos Eduardo da Silveira [UNIFESP]
Sasaki, Pedro Henrique
Abdalla, Rene Jorge [UNIFESP]
Is part ofActa Ortopédica Brasileira
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OBJECTIVE:This study is to report a manual method to obtain platelet rich plasma (PRP).METHODS:For this study 61 ml of peripheral blood was obtained and submitted to centrifugation at 541g for 5 min. The centrifugation separates the blood into three components: red blood cells, buffy coat and platelet rich plasma. Blood and platelet rich plasma samples were sent to the Hospital's Laboratory and platelets and leukocytes were measured.RESULTS:A sample of 637 blood donors was evaluated. The platelet yield efficiency was 86.77% and the increase in platelet concentration factor was 2.89 times. The increase in leukocyte concentration factor was 1.97 times.CONCLUSION:The method described here produces leukocyte-rich and platelet-rich plasma with a high platelet and leukocyte increased factor.Level of Evidence IV, Controlled Laboratory Study.
CitationActa Ortopédica Brasileira. Sociedade Brasileira de Ortopedia e Traumatologia, v. 22, n. 2, p. 75-77, 2014.
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