APC protein immunoexpression in colorectal adenoma and adenocarcinoma

APC protein immunoexpression in colorectal adenoma and adenocarcinoma

Alternative title Imunoexpressao da proteina APC nos tecidos de adenoma e de adenocarcinoma colorretais
Author Bourroul, Vivian Sati Oba Google Scholar
Bourroul, Guilherme Muniz Google Scholar
Toloi, Giovanna Canato Google Scholar
Palma, Rogerio Tadeu Google Scholar
Oshima, Celina Tizuko Fujiyama Autor UNIFESP Google Scholar
Gomes, Thiago Simao Autor UNIFESP Google Scholar
Ihara, Silvia Saiuli Miki Autor UNIFESP Google Scholar
Waisberg, Jaques Google Scholar
Institution Hospital do Servidor Publico Estadual Service of Digestive System Surgery
Hospital Estadual Mario Covas Service of Digestive System Surgery
Faculdade de Medicina do ABC
Faculdade de Medicina do ABC Service of Digestive System Surgery
Universidade Federal de São Paulo (UNIFESP)
Faculdade de Medicina do ABC General and Digestive System Surgery
Abstract BACKGROUND:activation of the Wnt pathway by mutated APC gene is considered the initial event in colorectal carcinogenesis. The identification of these mutations can improve the specific treatment of the adenocarcinoma.OBJECTIVE:detect and evaluate wild-type APC protein in tissue from colorectal adenoma, adenocarcinoma and adjacent mucosa.METHODS:42 patients that underwent surgery for adenocarcinoma and 53 patients with resected adenomas were studied. Tissue samples from the adenocarcinoma were obtained from the tumor and from adjacent non-neoplastic mucosa located 10 cm from the proximal margin of the tumor. Adenoma tissue was obtained from representative areas. Blocks of tissue microarray (TMA) were submitted to immunohistochemistry with anti-APC, with readings of positivity and intensity of immunostaining and the score of immune expression of APC protein was obtained.RESULTS:the APC protein immune expression score showed a significantly lower expression of APC protein in the adenoma when compared with the adenocarcinoma (p < 0.0001) and adjacent mucosa (p < 0.0001). The APC protein immune expression score in the colorectal mucosa and adjacent to the adenocarcinoma showed no significant difference (p = 0.24).CONCLUSIONS:the finding of decreased expression of APC protein in adenoma tissue may indicate that the mutated APC gene may contribute to the changes in the adenoma-carcinoma process of carcinogenesis sequence. The strong expression of protein APC in tissues from the carcinoma and adjacent mucosa suggests that in most patients in this series, the mutation of the APC gene did not participate in the oncogenesis mechanism.

RACIONAL:a ativação da via Wnt pelo gene APC mutado é considerado evento inicial da carcinogênese colorretal. A identificação dessas mutações pode tornar o tratamento do adenocarcinoma mais específico.OBJETIVO:detectar e avaliar a proteína APC não mutada em tecidos de adenoma, adenocarcinoma e mucosa adjacente.MÉTODO:estudados 42 doentes operados de adenocarcinoma e 53 com adenomas ressecados. Tecidos de adenocarcinoma foram obtidas da neoplasia e da mucosa adjacente não neoplásica situadas a 10 cm da margem proximal do tumor. Tecidos do adenoma foram obtidas de área representativa. Blocos de tissue microarray (TMA) foram submetidos a imuno-histoquímica com anticorpo anti-APC. Avaliadas a positividade e intensidade da expressão e obtidos escores da imunoexpressão da proteína APC.RESULTADOS:o escore da imunoexpressão da proteína APC no adenoma foi significantemente menor do que no adenocarcinoma (p < 0,0001) e na mucosa adjacente (p < 0,0001). O escore da imunoexpressão da proteína APC na mucosa adjacente e no adenocarcinoma não mostraram diferença significante (p = 0,24).CONCLUSÕES:a menor expressão da proteína APC no adenoma pode indicar que o gene APC mutado participa das alterações do processo adenoma-carcinoma. A forte expressão da proteína APC no CCR e na mucosa adjacente sugerem que a mutação do gene APC não participou da oncogênese.
Keywords Colorectal neoplasms
APC Gene
Biological tumor markers
Neoplasias colorretais
Gene APC
Marcadores biologicos de tumor
Language English
Date 2013-09-01
Published in Journal of Coloproctology (Rio de Janeiro). Sociedade Brasileira de Coloproctologia, v. 33, n. 3, p. 118-125, 2013.
ISSN 2237-9363 (Sherpa/Romeo)
Publisher Sociedade Brasileira de Coloproctologia
Extent 118-125
Origin http://dx.doi.org/10.1016/j.jcol.2013.06.002
Access rights Open access Open Access
Type Article
SciELO ID S2237-93632013000300118 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/7920

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