Evaluation of rapid tests for human immunodeficiency virus as a tool to detect recent seroconversion

Evaluation of rapid tests for human immunodeficiency virus as a tool to detect recent seroconversion

Author Girardi, Samira Barboza Autor UNIFESP Google Scholar
Barreto, Angela Maria Egydio de Carvalho Google Scholar
Barreto, Claudia Cortese Google Scholar
Proietti, Anna Barbara Google Scholar
Carvalho, Silvia Maia Farias de Google Scholar
Loureiro, Paula Google Scholar
Sabino, Ester Cerdeira Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Fundação Pró-Sangue Hemocentro de São Paulo
Universidade de São Paulo (USP)
Fundação Centro de Hematologia e Hemoterapia de Minas Gerais
Faculdade de Saúde e Ecologia Humana
Instituto Estadual de Hematologia Arthur de Siqueira Cavalcanti
Fundação Hemope
Abstract The identification of recent HIV infection is important for epidemiological studies and to monitor the epidemic. The objective of this study was to evaluate two rapid tests that are easily available to the Brazilian scientific community for using as markers of recent HIV infection. The Rapid Test - HIV-1/2 Bio-Manguinhos (Bio-Manguinhos/Fiocruz, Brazil) and the Rapid Check HIV 1&2 (NDI-UFES, Center for Infectious Diseases, Universidade Federal do Espírito Santo) were tested, using 489 samples with HIV positive serology, from blood donors, previously classified as recent or long-term infection by serological testing algorithm for recent HIV seroconversion (STARHS) or LS-HIV Vitros assay methods. The samples were diluted prior to testing (1:50 and 1:100 for the Rapid Test - HIV-1/2 Bio-Manguinhos, and 1:500 and 1:600 for the Rapid Check HIV 1&2). Negative samples were considered recent infection, whereas those showing any color intensity were associated with long-term infection. The best dilutions were 1:100 for HIV-1/2 Bio-Manguinhos test (Kappa = 0.840; overall agreement = 0.93), and 1:500 for the Rapid Check HIV 1&2 (Kappa = 0.867; overall agreement = 0.94). The results suggest that both rapid tests can be used to detect recent seroconversion.
Keywords HIV-1 incidence
Detuned assay
Rapid test
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2012-10-01
Published in Brazilian Journal of Infectious Diseases. Brazilian Society of Infectious Diseases, v. 16, n. 5, p. 452-456, 2012.
ISSN 1413-8670 (Sherpa/Romeo, impact factor)
Publisher Brazilian Society of Infectious Diseases
Extent 452-456
Origin http://dx.doi.org/10.1016/j.bjid.2012.08.013
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000310088400009
SciELO ID S1413-86702012000500009 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/7331

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