Influence of oxidative stress on inducing micturition dysfunction following chronic infravesical obstruction and the protective role of an antioxidant diet - association of in vivo and in vitro studies in rats

Influence of oxidative stress on inducing micturition dysfunction following chronic infravesical obstruction and the protective role of an antioxidant diet - association of in vivo and in vitro studies in rats

Author Bisogni, Sérgio Google Scholar
Ferreira, Fabio Thadeu Autor UNIFESP Google Scholar
Amstalden Neto, Arnaldo Autor UNIFESP Google Scholar
Chiarelli, Leandro Oliveira Autor UNIFESP Google Scholar
Ortiz, Valdemar Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract OBJECTIVE: This study was developed to determine whether the generation of free radicals, induced by ischemia followed by reperfusion in a model of chronic intravesical obstruction in rats, would lead to damage in the detrusor. It also investigates the possible protective action of the flavonoid galangin on the tissue lesion induced by lipid peroxidation. MATERIALS AND METHODS: Twenty-one male rats were divided into three groups of seven animals each. Group A was subjected to a sham procedure; group B to partial obstruction of the bladder neck; and group C to partial obstruction of the bladder neck, but also received a diet rich in the flavonoid galangin. All the animals were subjected to urodynamic evaluation and then sacrificed. The bladders were sent for enzymatic tests. RESULTS: The urodynamic showed that group B developed significantly greater numbers of involuntary contractions of the detrusor, greater post-micturition residue and lower compliance. The group A presented TEAC levels greater than to the group B. Comparative analysis of group A, B and C demonstrated significantly greater malondialdehyde levels in group B in relation to groups A and C. The group B presented smaller contraction amplitudes than did groups A and C, in electrically stimulated contractions. CONCLUSIONS: That oxidative stress is implicated in the damage to the detrusor musculature following a period of chronic intravesical obstruction. We show, for the first time, that administration of an antioxidant prior to and following the start of chronic obstruction makes it possible to avoid the cellular lesions that cause detrusor dysfunction.
Keywords Urinary Bladder
Oxidative stress
Muscle Contraction
Flavonoids
Language English
Date 2012-08-01
Published in International braz j urol. Sociedade Brasileira de Urologia, v. 38, n. 4, p. 552-560, 2012.
ISSN 1677-5538 (Sherpa/Romeo, impact factor)
Publisher Sociedade Brasileira de Urologia
Extent 552-560
Origin http://dx.doi.org/10.1590/S1677-55382012000400016
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000314418700019
SciELO ID S1677-55382012000400016 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/7211

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