Detection of adrenocortical autoantibodies in Addison's disease with a peroxidase-labelled protein A technique

Show simple item record

dc.contributor.author Silva, Reinaldo Correia [UNIFESP]
dc.contributor.author Faiçal, Samir [UNIFESP]
dc.contributor.author Laureti, Stefano
dc.contributor.author Falorni, Alberto
dc.contributor.author Dib, Sergio Atala [UNIFESP]
dc.contributor.author Kater, Claudio Elias [UNIFESP]
dc.date.accessioned 2015-06-14T13:24:46Z
dc.date.available 2015-06-14T13:24:46Z
dc.date.issued 1998-09-01
dc.identifier http://dx.doi.org/10.1590/S0100-879X1998000900007
dc.identifier.citation Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 31, n. 9, p. 1141-1148, 1998.
dc.identifier.issn 0100-879X
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/674
dc.description.abstract Adrenocortical autoantibodies (ACA), present in 60-80% of patients with idiopathic Addison's disease, are conventionally detected by indirect immunofluorescence (IIF) on frozen sections of adrenal glands. The large-scale use of IIF is limited in part by the need for a fluorescence microscope and the fact that histological sections cannot be stored for long periods of time. To circumvent these restrictions we developed a novel peroxidase-labelled protein A (PLPA) technique for the detection of ACA in patients with Addison's disease and compared the results with those obtained with the classical IIF assay. We studied serum samples from 90 healthy control subjects and 22 patients with Addison's disease, who had been clinically classified into two groups: idiopathic (N = 13) and granulomatous (N = 9). ACA-PLPA were detected in 10/22 (45%) patients: 9/13 (69%) with the idiopathic form and 1/9 (11%) with the granulomatous form, whereas ACA-IIF were detected in 11/22 patients (50%): 10/13 (77%) with the idiopathic form and 1/9 (11%) with the granulomatous form. Twelve of the 13 idiopathic addisonians (92%) were positive for either ACA-PLPA or ACA-IIF, but only 7 were positive by both methods. In contrast, none of 90 healthy subjects was found to be positive for ACA. Thus, our study shows that the PLPA-based technique is useful, has technical advantages over the IIF method (by not requiring the use of a fluorescence microscope and by permitting section storage for long periods of time). However, since it is only 60% concordant with the ACA-IIF method, it should be considered complementary instead of an alternative method to IIF for the detection of ACA in human sera. en
dc.format.extent 1141-1148
dc.language.iso eng
dc.publisher Associação Brasileira de Divulgação Científica
dc.relation.ispartof Brazilian Journal of Medical and Biological Research
dc.rights Acesso aberto
dc.subject autoantibodies detection en
dc.subject Addison's disease en
dc.subject peroxidase-labelled protein A en
dc.subject indirect immunofluorescence en
dc.subject adrenal gland en
dc.title Detection of adrenocortical autoantibodies in Addison's disease with a peroxidase-labelled protein A technique en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution University of Perugia
dc.description.affiliation Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation University of Perugia
dc.description.affiliationUnifesp UNIFESP
dc.identifier.file S0100-879X1998000900007.pdf
dc.identifier.scielo S0100-879X1998000900007
dc.identifier.doi 10.1590/S0100-879X1998000900007
dc.description.source SciELO
dc.identifier.wos WOS:000076297100007



File

Name: S0100-879X1998000900007.pdf
Size: 58.07Kb
Format: PDF
Description:
Open file

This item appears in the following Collection(s)

Show simple item record

Search


Browse

Statistics

My Account