Detection of adrenocortical autoantibodies in Addison's disease with a peroxidase-labelled protein A technique

Detection of adrenocortical autoantibodies in Addison's disease with a peroxidase-labelled protein A technique

Author Silva, Reinaldo Correia Autor UNIFESP Google Scholar
Faiçal, Samir Autor UNIFESP Google Scholar
Laureti, Stefano Google Scholar
Falorni, Alberto Google Scholar
Dib, Sergio Atala Autor UNIFESP Google Scholar
Kater, Claudio Elias Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
University of Perugia
Abstract Adrenocortical autoantibodies (ACA), present in 60-80% of patients with idiopathic Addison's disease, are conventionally detected by indirect immunofluorescence (IIF) on frozen sections of adrenal glands. The large-scale use of IIF is limited in part by the need for a fluorescence microscope and the fact that histological sections cannot be stored for long periods of time. To circumvent these restrictions we developed a novel peroxidase-labelled protein A (PLPA) technique for the detection of ACA in patients with Addison's disease and compared the results with those obtained with the classical IIF assay. We studied serum samples from 90 healthy control subjects and 22 patients with Addison's disease, who had been clinically classified into two groups: idiopathic (N = 13) and granulomatous (N = 9). ACA-PLPA were detected in 10/22 (45%) patients: 9/13 (69%) with the idiopathic form and 1/9 (11%) with the granulomatous form, whereas ACA-IIF were detected in 11/22 patients (50%): 10/13 (77%) with the idiopathic form and 1/9 (11%) with the granulomatous form. Twelve of the 13 idiopathic addisonians (92%) were positive for either ACA-PLPA or ACA-IIF, but only 7 were positive by both methods. In contrast, none of 90 healthy subjects was found to be positive for ACA. Thus, our study shows that the PLPA-based technique is useful, has technical advantages over the IIF method (by not requiring the use of a fluorescence microscope and by permitting section storage for long periods of time). However, since it is only 60% concordant with the ACA-IIF method, it should be considered complementary instead of an alternative method to IIF for the detection of ACA in human sera.
Keywords autoantibodies detection
Addison's disease
peroxidase-labelled protein A
indirect immunofluorescence
adrenal gland
Language English
Date 1998-09-01
Published in Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 31, n. 9, p. 1141-1148, 1998.
ISSN 0100-879X (Sherpa/Romeo, impact factor)
Publisher Associação Brasileira de Divulgação Científica
Extent 1141-1148
Origin http://dx.doi.org/10.1590/S0100-879X1998000900007
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000076297100007
SciELO ID S0100-879X1998000900007 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/674

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