Trypanosoma cruzi trypomastigotes induce cytoskeleton modifications during HeLa cell invasion
Fernandes, Maria Cecília
Andrade, Leonardo Rodrigues De
Andrews, Norma Windsor
Mortara, Renato Arruda [UNIFESP]
Is part ofMemórias do Instituto Oswaldo Cruz
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It has been recently shown that Trypanosoma cruzi trypomastigotes subvert a constitutive membrane repair mechanism to invade HeLa cells. Using a membrane extraction protocol and high-resolution microscopy, the HeLa cytoskeleton and T. cruzi parasites were imaged during the invasion process after 15 min and 45 min. Parasites were initially found under cells and were later observed in the cytoplasm. At later stages, parasite-driven protrusions with parallel filaments were observed, with trypomastigotes at their tips. We conclude that T. cruzi trypomastigotes induce deformations of the cortical actin cytoskeleton shortly after invasion, leading to the formation of pseudopod-like structures.
CitationMemórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 106, n. 8, p. 1014-1016, 2011.
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