Prospecção dos efeitos da administração do quiabo e/ou treinamento físico em ratos com síndrome metabólica
Data
2021-10-27
Autores
Luna, Moisés Felipe Pereira Gomes de [UNIFESP]
Orientadores
Medeiros, Alessandra [UNIFESP]
Tipo
Tese de doutorado
Título da Revista
ISSN da Revista
Título de Volume
Resumo
A síndrome metabólica (SM) consiste em um fenômeno clínico caracterizado por um conjunto de fatores de riscos para o desenvolvimento de doenças cardiovasculares. Apesar de nos últimos anos, o extrato de quiabo ter sido estudado no tratamento dos componentes da SM, até o momento nenhum estudo analisou o efeito do consumo de quiabo na SM per se ou os efeitos do quiabo no tecido cardíaco de ratos com SM. Portanto, o objetivo do presente estudo foi avaliar o efeito do consumo de quiabo e/ou treinamento físico (TF) em animais com SM. Ao todo, 40 ratos Zucker (fa/fa) foram randomicamente separados em quatro grupos de 10 animais cada: SM; Quiabo (Q); TF; TF mais Quiabo (TFQ), e 10 Zucker lean (fa/+) compuseram o grupo Controle (CTL). O quiabo foi administrado na concentração de 100 mg/kg, 2x/dia, 5 dias/semana, durante as 6 semanas. O TF foi realizado em esteira rolante, 5 dias/semana, 60 minutos/dia, com intensidade de 70% da velocidade obtida no teste de esforço máximo, durante as 6 semanas. Na sexta semana foram realizadas avaliação dos componentes da SM: triacilglicerol (TG), colesterol total, pressão arterial (PA), massa corporal e o teste de sensibilidade à insulina intraperitoneal (ipITT) Após a eutanásia foram realizadas as seguintes avaliações nos tecidos cardíacos e hepáticos: Elisa das interleucinas 6, 10 e fator de necrose tumoral-α (TNF-α); substâncias reativas ao ácido tiobarbitúrico (TBARS), atividade oxidante e antioxidante. Além disso, foi estimado o glicogênio hepático e realizada análise histológica do ventrículo esquerdo. Apesar do consumo de Q, não ter reduzido a massa corpórea, TNF-α, MDA, capacidade oxidante total (TOS) e ter aumentado os níveis de IL-10 e capacidade antixiodante total (TAS) no tecido cardíaco. Por outro lado, o consumo de Q promoveu: redução da resistência à insulina (RI), reduziu os níveis de IL-6 (CTL = 67,9 ± 8,0 pg/µg; SM = 84,7 ± 27,5 pg/µg; Q = 28,4 ± 8,0 pg/µg; TF = 37,3 ± 22,6 pg/µg e TFQ = 45,5 ± 24,8 pg/µg), redução da fração de colágeno cardíaco (CTL = 6,68 ± 2,25%; SM = 12,60 ± 2,08%; Q = 7,52 ± 0,77%; TF = 14,48 ± 2,35% e TFQ = 5,66 ± 0,34%), aumento do diâmetro dos cardiomiócitos (CTL = 13935 ± 2821 µm; SM = 14341 ± 2824 µm; Q = 15570 ± 2626 µm; TF = 14584 ± 2355 µm e TFQ = 15146 ± 2786 µm), redução da PA sistólica (CTL = 131,3 ± 2,9; SM = 149,0 ± 9,3 mmHg; Q = 132,0 ± 11,4 mmHg; TF = 129,2 ± 14,9 mmHg e TFQ = 135,2 ± 5,4 mmHg), e do TG (CTL = 92,45 ± 18,8 mg/dL; SM = 492,9 ± 97,8 mg/dL; Q = 334,9 ± 98,0 mg/dL; TF = 465,0 ± 113,3 mg/dL e TFQ = 412,0 ± 54,4 mg/dL). No tecido hepático, notamos que o consumo de Q não teve interferência nos níveis de IL-10, TNF-α e MDA. No entanto, promoveu maior concentração de glicogênio hepático CTL = 2.675 ± 103,0 µg/g; SM = 2213 ± 514,3 µg/g; Q = 5488 ± 1746 µg/g; TF 7024 ± 2594 µg/g e TFQ 5708 ± 1299 µg/g), e quando associado com o TF promoveu redução da IL-6 (CTL = 12,2 ± 1,1 pg/µg; SM = 48,4 ± 6,0 pg/µg; Q = 44,0 ± 8,5 pg/µg; TF = 39,4 ± 9,0 pg/µg; TFQ = 22,9 ± 7,7 pg/µg) e do TOS (CTL = 19,3 ± 5,8; SM = 18,3 ± 5,8 Q = 14,0 ± 9,8; TF = 16,8 ± 3,1 e TFQ = 3,6 ± 0,1). Dessa forma, conclui-se que o consumo de Q associado ou não ao TF pode exercer efeito protetor do tecido cardíaco de ratos com SM.
The Metabolic Syndrome (MetS) is a clinical manifestation associated with cardiovascular disease. Although in the past few years, okra extract has been studied in the treatment of the components of MetS, so far no study has analyzed the effect of okra consumption on MetS or the effects of okra on the cardiac tissue of rats with MetS. Therefore, the present study the aim was to evaluate the effect of okra consumption and/or physical training in animals with MetS. In all, 40 Zucker rats (fa/fa) were randomly distributed into four groups of 10 animals each: Metabolic (MetS); Okra (O); PT and Physical Training and Okra (PTO), and 10 lean Zucker rats (fa/+) comprised the control group (CTL). Okra was administered at a concentration of 200mg/kg, 5 days/week, for 6 weeks. Physical training (PT) was performed on a treadmill, 5 days/week, 60 minutes/day, in an intensity of 70% of the speed obtained in the maximum effort test, along the 6 weeks. At week 6, MetS components were evaluated: triglyceride (TG), total cholesterol, blood pressure (BP), body mass and the intraperitoneal insulin sensitivity test (ipITT). After euthanasia, the following assessments were performed on cardiac and hepatic tissues: Elisa of interleukins 6, 10 and tumor necrosis factor-α; substances reactive to thiobarbituric acid (TBARS), oxidizing and antioxidant activity. Despite O consumption, it did not reduce body mass, TNF-α, MDA, total oxidizing capacity (TOS) and increased IL-10 levels and total antioxidant capacity (TAS) in cardiac tissue. On the other hand, Q consumption promoted: improvement in insulin resistance (RI), reduced IL-6 levels (CTL = 67.9 ± 8.0 pg/µg; MetS = 84.7 ± 27.5 pg /µg; O = 28.4 ± 8.0 pg/µg; PT = 37.3 ± 22.6 pg/µg and PTO = 45.5 ± 24.8 pg/µg), reduction in cardiac collagen fraction ( CTL = 6.68 ± 2.25%; MetS = 12.60 ± 2.08%; O = 7.52 ± 0.77%; PT = 14.48 ± 2.35% and PTO = 5.66 ± 0.34%), increased diameter of cardiomyocytes (CTL = 13935 ± 2821 µm; MetS = 14341 ± 2824 µm; O = 15570 ± 2626 µm; PT = 14584 ± 2355 µm and PTO = 15146 ± 2786 µm), reduction of Systolic BP (CTL = 131.3 ± 2.9; MetS = 149.0 ± 9.3 mmHg; O = 132.0 ± 11.4 mmHg; PT = 129.2 ± 14.9 mmHg and PTO = 135, 2 ± 5.4 mmHg), and TG (CTL = 92.45 ± 18.8 mg/dL; MetS = 492.9 ± 97.8 mg/dL; O = 334.9 ± 98.0 mg/dL; PT = 465.0 ± 113.3 mg/dL and PTO = 412.0 ± 54.4 mg/dL). In liver tissue, we noticed that the consumption of O had no interference in the levels of IL-10, TNF-α and MDA. However, it promoted a higher concentration of hepatic glycogen CTL = 2,675 ± 103.0 µg/g; MetS = 2213 ± 514.3 µg/g; O = 5488 ± 1746 µg/g; PT 7024 ± 2594 µg/g and PTO 5708 ± 1299 µg/g), and when associated with PT it promoted a reduction in IL-6 (CTL = 12.2 ± 1.1 pg/µg; MetS = 48.4 ± 6, 0 pg/µg; O = 44.0 ± 8.5 pg/µg; PT = 39.4 ± 9.0 pg/µg; PTO = 22.9 ± 7.7 pg/µg) and the TOS (CTL = 19.3 ± 5.8; MetS = 18.3 ± 5.8 O = 14.0 ± 9.8; PT = 16.8 ± 3.1 and PTO = 3.6 ± 0.1). Thus, it is concluded that the consumption of O associated or not with PT can have a protective effect on the cardiac tissue of rats with MetS
The Metabolic Syndrome (MetS) is a clinical manifestation associated with cardiovascular disease. Although in the past few years, okra extract has been studied in the treatment of the components of MetS, so far no study has analyzed the effect of okra consumption on MetS or the effects of okra on the cardiac tissue of rats with MetS. Therefore, the present study the aim was to evaluate the effect of okra consumption and/or physical training in animals with MetS. In all, 40 Zucker rats (fa/fa) were randomly distributed into four groups of 10 animals each: Metabolic (MetS); Okra (O); PT and Physical Training and Okra (PTO), and 10 lean Zucker rats (fa/+) comprised the control group (CTL). Okra was administered at a concentration of 200mg/kg, 5 days/week, for 6 weeks. Physical training (PT) was performed on a treadmill, 5 days/week, 60 minutes/day, in an intensity of 70% of the speed obtained in the maximum effort test, along the 6 weeks. At week 6, MetS components were evaluated: triglyceride (TG), total cholesterol, blood pressure (BP), body mass and the intraperitoneal insulin sensitivity test (ipITT). After euthanasia, the following assessments were performed on cardiac and hepatic tissues: Elisa of interleukins 6, 10 and tumor necrosis factor-α; substances reactive to thiobarbituric acid (TBARS), oxidizing and antioxidant activity. Despite O consumption, it did not reduce body mass, TNF-α, MDA, total oxidizing capacity (TOS) and increased IL-10 levels and total antioxidant capacity (TAS) in cardiac tissue. On the other hand, Q consumption promoted: improvement in insulin resistance (RI), reduced IL-6 levels (CTL = 67.9 ± 8.0 pg/µg; MetS = 84.7 ± 27.5 pg /µg; O = 28.4 ± 8.0 pg/µg; PT = 37.3 ± 22.6 pg/µg and PTO = 45.5 ± 24.8 pg/µg), reduction in cardiac collagen fraction ( CTL = 6.68 ± 2.25%; MetS = 12.60 ± 2.08%; O = 7.52 ± 0.77%; PT = 14.48 ± 2.35% and PTO = 5.66 ± 0.34%), increased diameter of cardiomyocytes (CTL = 13935 ± 2821 µm; MetS = 14341 ± 2824 µm; O = 15570 ± 2626 µm; PT = 14584 ± 2355 µm and PTO = 15146 ± 2786 µm), reduction of Systolic BP (CTL = 131.3 ± 2.9; MetS = 149.0 ± 9.3 mmHg; O = 132.0 ± 11.4 mmHg; PT = 129.2 ± 14.9 mmHg and PTO = 135, 2 ± 5.4 mmHg), and TG (CTL = 92.45 ± 18.8 mg/dL; MetS = 492.9 ± 97.8 mg/dL; O = 334.9 ± 98.0 mg/dL; PT = 465.0 ± 113.3 mg/dL and PTO = 412.0 ± 54.4 mg/dL). In liver tissue, we noticed that the consumption of O had no interference in the levels of IL-10, TNF-α and MDA. However, it promoted a higher concentration of hepatic glycogen CTL = 2,675 ± 103.0 µg/g; MetS = 2213 ± 514.3 µg/g; O = 5488 ± 1746 µg/g; PT 7024 ± 2594 µg/g and PTO 5708 ± 1299 µg/g), and when associated with PT it promoted a reduction in IL-6 (CTL = 12.2 ± 1.1 pg/µg; MetS = 48.4 ± 6, 0 pg/µg; O = 44.0 ± 8.5 pg/µg; PT = 39.4 ± 9.0 pg/µg; PTO = 22.9 ± 7.7 pg/µg) and the TOS (CTL = 19.3 ± 5.8; MetS = 18.3 ± 5.8 O = 14.0 ± 9.8; PT = 16.8 ± 3.1 and PTO = 3.6 ± 0.1). Thus, it is concluded that the consumption of O associated or not with PT can have a protective effect on the cardiac tissue of rats with MetS
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Citação
GOMES, Moisés Felipe Pereira Gomes de. Prospecção dos efeitos da administração do quiabo e/ou treinamento físico em ratos com síndrome metabólica. 2021. 74 f. Tese (Doutorado Interdisciplinar em Ciências da Saúde) - Instituto de Saúde e Sociedade, Universidade Federal de São Paulo. Santos, 2021.