Efeito da exposição ao material particulado inalável fino (MP2,5) presente no ar da cidade de São Paulo na qualidade funcional dos espermatozoides de camundongos.
Data
2021-12-15
Autores
Tamashiro, Leticia Kaory 
Orientadores
Lopes, Paula Intasqui
Tipo
Dissertação de mestrado
Título da Revista
ISSN da Revista
Título de Volume
Resumo
Objetivo: Avaliar experimentalmente o efeito da exposição ao material particulado inalável fino (MP2,5) presente no ar da cidade de São Paulo na qualidade funcional dos espermatozoides de camundongos. Métodos: Foram utilizados 14 camundongos machos BALB/c isogênicos distribuídos em dois grupos, controle (n=6) e poluição (n=8). Para o grupo poluição, após o desmame (21 dias), os animais foram diariamente expostos a altas concentações do MP2,5 (600 µg/m3) durante 96 dias no Concentrador de Partículas Ambientais. O grupo controle foi exposto simultaneamente ao ar filtrado em uma câmara paralela. Após, os animais foram eutanasiados com overdose de isoflurano, o peso corporal foi avaliado e foi feita a coleta cirúrgica dos epidídimos. Espermatozoides obtidos da cauda dos epidídimos foram utilizados para as análises de integridade funcional (motilidade, viabilidade, integridade do acrossoma e atividade mitocondrial), de fragmentação de DNA e da produção intracelular de espécies reativas de oxigênio. Para a análise estatística, foi verificada a normalidade da distribuição dos dados pelo teste de Kolmogorov-Smirnov. Então, os grupos controle e poluição foram comparados pelo teste t de Student para amostras independentes e um erro alfa de 5% foi adotado. Resultados: No presente estudo, não foram observadas diferenças estatisticamente significativas entre os grupos controle e poluição em relação ao peso corporal e à integridade funcional, fragmentação do DNA e produção de espécies reativas de oxigênio nos espermatozoides. Conclusão: A exposição a altas concentrações de MP2,5 não provocou alterações nas variáveis espermáticas avaliadas neste estudo.
Objective: To evaluate the effect of the exposure to inhalable fine particulate matter (PM2.5) present in the air of the city of São Paulo on mice sperm functional quality. Methods: Fourteen male BALB/c isogenic mice were distributed into two groups, control (n=6) and pollution (n=8). For the pollution group, after weaning (21 days), animals were daily exposed to high concentrations of PM2.5 (600 µg/m3) for 96 days in the Ambient Particle Concentrator. The control group was simultaneously exposed to filtered air in a parallel chamber. After exposure period, animals were euthanized with an isoflurane overdose, body was weighted and the epididymis were collected. Sperm obtained from the cauda epididymis were used for the evaluation of functional integrity (motility, viability, acrosome integrity and mitochondrial activity), DNA fragmentation and intracellular production of reactive oxygen species. For statistical analysis, the normality of data distribution was verified by the Kolmogorov-Smirnov test. Then, control and pollution groups were compared by an unpaired Student's t test with an alpha of 5%. Results: In the present study, no statistically significant differences were observed between the control and pollution groups in relation to body weight and sperm functional integrity, DNA fragmentation and production of reactive oxygen species. Conclusion: Exposure to high concentrations of PM2.5 did not change sperm variables evaluated in this study.
Objective: To evaluate the effect of the exposure to inhalable fine particulate matter (PM2.5) present in the air of the city of São Paulo on mice sperm functional quality. Methods: Fourteen male BALB/c isogenic mice were distributed into two groups, control (n=6) and pollution (n=8). For the pollution group, after weaning (21 days), animals were daily exposed to high concentrations of PM2.5 (600 µg/m3) for 96 days in the Ambient Particle Concentrator. The control group was simultaneously exposed to filtered air in a parallel chamber. After exposure period, animals were euthanized with an isoflurane overdose, body was weighted and the epididymis were collected. Sperm obtained from the cauda epididymis were used for the evaluation of functional integrity (motility, viability, acrosome integrity and mitochondrial activity), DNA fragmentation and intracellular production of reactive oxygen species. For statistical analysis, the normality of data distribution was verified by the Kolmogorov-Smirnov test. Then, control and pollution groups were compared by an unpaired Student's t test with an alpha of 5%. Results: In the present study, no statistically significant differences were observed between the control and pollution groups in relation to body weight and sperm functional integrity, DNA fragmentation and production of reactive oxygen species. Conclusion: Exposure to high concentrations of PM2.5 did not change sperm variables evaluated in this study.