Bioconversion of low-cost brewery waste to biosurfactant: an improvement of surfactin production by culture medium optimization
Corrêa Nazareth, Talita
Planas Zanutto, Conrado
Maass, Danielle [UNIFESP]
Ulson de Souza, Antônio Augusto
Guelli Ulson de Souza, Selene Maria de Arruda
Is part ofBiochemical Engineering Journal
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Alternative TitleBioconversion of low-cost brewery waste to biosurfactant: an improvement of surfactin production by culture medium optimization
Trub is the second-largest residue from brewing industry (250 thousand tons/year) and, due to the presence of high amounts of carbon and nitrogen, it is a potential source of reducing sugars and nitrogen for biological processes. Therefore, this work aimed to enhance surfactin production by B. subtilis using Trub as a carbon source. The effect of nitrogen sources on biosurfactant production was investigated by a Full Factorial Design (2²). Yeast extract and its interaction with peptone presented a statistically significant effect on the response variable of surface tension (ST). ST was described by a linear regression model (R² = 0.93). The effect of metals supplementation on the culture medium was also investigated, where the volumetric productivity increased from 2.98 to 7.43 mg.L-1.h-1 for the medium without (M-I) and with metals (M-II), respectively. After 28h, 210.11 mg.L-1 was obtained in the batch culture with MII medium, being higher than the maximum surfactin concentration achieved after 52 h (121.80 mg.L-1) for M-I medium. The biosurfactant production in bioreactor was successfully performed since a concentrated extract was obtained in the foam collected, reaching the maximum of 1118.20 mg.L-1 of surfactin.
CitationTalita Corrêa Nazareth; Conrado Planas Zanutto; Danielle Maass; Antônio Augusto Ulson de Souza; Selene Maria de Arruda Guelli Ulson de Souza. Bioconversion of low-cost brewery waste to biosurfactant: An improvement of surfactin production by culture medium optimization, Biochemical Engineering Journal, v. 172, 2021, 108058, ISSN 1369-703X.
SponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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