Raising the Bar(-seq) in Leishmania genetic screens

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Date
2021
Author
Ferreira, Tiago R.
Couñago, Rafael M.
Moretti, Nilmar Silvio [UNIFESP]
Type
Artigo
Is part of
Trends in Parasitology
DOI
https://doi.org/10.1016/j.pt.2021.03.002
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Abstract
Our understanding of regulatory factors in Leishmania differentia- tion has long been restricted by the available genetic tools, but the availability of CRISPR/Cas9 has changed the landscape forever. Recently, Baker and Catta-Preta et al. applied Cas9 editing and kinome-wide bar-seq to dissect the function of 204 kinases in the Leishmania mexicana life cycle.
Keywords
Leishmaniasis
Kinase
CRISPR-Cas9
Sponsorship
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
URI
https://repositorio.unifesp.br/xmlui/handle/11600/62325
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