Raising the Bar(-seq) in Leishmania genetic screens

View/ Open
Date
2021Author
Ferreira, Tiago R.
Couñago, Rafael M.
Moretti, Nilmar Silvio [UNIFESP]
Type
ArtigoIs part of
Trends in ParasitologyDOI
https://doi.org/10.1016/j.pt.2021.03.002Metadata
Show full item recordAbstract
Our understanding of regulatory factors in Leishmania differentia- tion has long been restricted by the available genetic tools, but the availability of CRISPR/Cas9 has changed the landscape forever. Recently, Baker and Catta-Preta et al. applied Cas9 editing and kinome-wide bar-seq to dissect the function of 204 kinases in the Leishmania mexicana life cycle.
Keywords
LeishmaniasisKinase
CRISPR-Cas9
Sponsorship
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Collections
- EPM - Artigos [16058]