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dc.contributor.authorde Jesus, Teresa Cristina Leandro
dc.contributor.authorNunes, Vinícius Santana [UNIFESP]
dc.contributor.authorLopes, Mariana de Camargo
dc.contributor.authorMartil, Daiana Evelin
dc.contributor.authorIwai, Leo Kei
dc.contributor.authorMoretti, Nilmar Silvio [UNIFESP]
dc.contributor.authorMachado, Fabrício Castro [UNIFESP]
dc.contributor.authorLima-Stein, Mariana L. de [UNIFESP]
dc.contributor.authorThiemann, Otavio Henrique
dc.contributor.authorElias, Maria Carolina
dc.contributor.authorJanzen, Christian
dc.contributor.authorSchenkman, Sergio [UNIFESP]
dc.contributor.authorCunha, Julia Pinheiro Chagas da
dc.date.accessioned2021-11-29T15:49:42Z
dc.date.available2021-11-29T15:49:42Z
dc.date.issued2016
dc.identifier.urihttps://repositorio.unifesp.br/xmlui/handle/11600/62324
dc.description.abstractHistones are well-conserved proteins that form the basic structure of chromatin in eukaryotes and undergo several post-translational modifications, which are important for the control of transcription, replication, DNA damage repair, and chromosome condensation. In early branched organisms, histones are less conserved and appear to contain alternative sites for modifications, which could reveal evolutionary unique functions of histone modifications in gene expression and other chromatin- based processes. Here, by using high-resolution mass spectrom- etry, we identified and quantified histone post-translational modifications in two life cycle stages of Trypanosoma cruzi, the protozoan parasite that causes Chagas disease. We detected 44 new modifications, namely: 18 acetylations, seven monomethylations, seven dimethylations, seven trimethylations, and four phosphorylations. We found that replicative (epimastigote stage) contains more histone modifications than nonreplicative and infective parasites (trypomastigote stage). Acetylations of lysines at the C-terminus of histone H2A and methylations of lysine 23 of histone H3 were found to be enriched in trypomastigotes. In contrast, phosphorylation in serine 23 of H2B and methylations of lysine 76 of histone H3 predominates in proliferative states. The presence of one or two methylations in the lysine 76 was found in cells undergoing mitosis and cytokinesis, typical of proliferating parasites. Our findings provide new insights into the role of histone modifications related to the control of gene expression and cell-cycle regulation in an early divergent organism.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.languageengpt_BR
dc.publisherACS Publicationspt_BR
dc.relation.ispartofJournal of Proteome Researchpt_BR
dc.rightsAcesso restritopt_BR
dc.subjectChromatinpt_BR
dc.subjectHistonept_BR
dc.subjectTrypanosoma cruzipt_BR
dc.subjectAcetylationpt_BR
dc.subjectMethylationpt_BR
dc.subjectCell cyclept_BR
dc.titleChromatin proteomics reveals variable histone modifications during the life cycle of Trypanosoma cruzipt_BR
dc.typeArtigopt_BR
dc.description.sponsorshipIDFAPESP: 2012/09403-8pt_BR
dc.identifier.doi10.1021/acs.jproteome.6b00208pt_BR
unifesp.campusEscola Paulista de Medicina (EPM)pt_BR
unifesp.graduateProgramMicrobiologia e Imunologiapt_BR
unifesp.knowledgeAreaOutrapt_BR
dc.contributor.authorLatteshttp://lattes.cnpq.br/2131472726202687pt_BR
unifesp.departamentoMicrobiologia, Imunologia e Parasitologiapt_BR


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