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dc.contributor.authorKeppeke, GD [UNIFESP]
dc.contributor.authorChang, CC
dc.contributor.authorAntos, CL
dc.contributor.authorPeng, M
dc.contributor.authorSung, LY
dc.contributor.authorAndrade, LEC [UNIFESP]
dc.contributor.authorLiu, JL
dc.date.accessioned2021-08-16T18:53:10Z
dc.date.available2021-08-16T18:53:10Z
dc.date.issued2021-05-25
dc.identifierhttps://doi.org/10.1016/j.ydbio.2021.05.017pt_BR
dc.identifier.citationPMID: 34048735pt_BR
dc.identifier.issn0012-1606pt_BR
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/61527
dc.descriptionAs figuras estão em acesso aberto.pt_BR
dc.description.abstractInosine monophosphate dehydrogenase (IMPDH) catalyzes the rate-limiting step in de novo guanine nucleotide biosynthesis. Its activity is negatively regulated by the binding of GTP. IMPDH can form a membraneless subcellular structure termed the cytoophidium in response to certain changes in the metabolic status of the cell. The polymeric form of IMPDH, which is the subunit of the cytoophidium, has been shown to be more resistant to the inhibition by GTP at physiological concentrations, implying a functional correlation between cytoophidium formation and the upregulation of GTP biosynthesis. Herein we demonstrate that zebrafish IMPDH1b and IMPDH2 isoforms can assemble abundant cytoophidium in most of cultured cells under stimuli, while zebrafish IMPDH1a shows distinctive properties of forming the cytoophidium in different cell types. Point mutations that disrupt cytoophidium structure in mammalian models also prevent the aggregation of zebrafish IMPDHs. In addition, we discover the presence of the IMPDH cytoophidium in various tissues of larval and adult fish under normal growth conditions. Our results reveal that polymerization and cytoophidium assembly of IMPDH can be a regulatory machinery conserved among vertebrates, and with specific physiological purposes.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.format.extent89-101pt_BR
dc.languageengpt_BR
dc.publisherOndine Cleaverpt_BR
dc.relation.ispartofDevelopmental Biologypt_BR
dc.rightsAcesso restritopt_BR
dc.subjectAdult zebrafishpt_BR
dc.subjectCytoophidiumpt_BR
dc.subjectIMPDHpt_BR
dc.subjectLarval zebrafishpt_BR
dc.titleIMPDH forms the cytoophidium in zebrafishpt_BR
dc.typeArtigopt_BR
dc.description.sponsorshipID2017/20745-1pt_BR
dc.identifier.doi10.1016/j.ydbio.2021.05.017pt_BR
unifesp.campusEscola Paulista de Medicina (EPM)pt_BR
dc.contributor.authorLatteshttp://lattes.cnpq.br/7269833349574761pt_BR
dc.citation.volume478pt_BR
unifesp.departamentoMedicinapt_BR
unifesp.assessoresproreitoriasPró-reitoria de Pós-graduação e Pesquisapt_BR


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