Marcadores analíticos para CLAE-DAD de extratos das folhas de Miconia albicans (SW) DC. (Melastomataceae)
Data
2021-07-30
Autores
Serafim, Ana Carolina Godonhoto [UNIFESP]
Orientadores
Andreo, Marcio Adriano [UNIFESP]
Tipo
Trabalho de conclusão de curso de graduação
Título da Revista
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Título de Volume
Resumo
Conhecida popularmente como canela-de-velho, Miconia albicans é utilizada como anti-inflamatória, sendo vendida livremente na forma de pomadas, cápsulas ou mesmo suas folhas secas ou moídas. Contudo, os estudos desta espécie são escassos. Sendo assim, este trabalho teve por objetivo isolar, purificar e identificar possíveis marcadores químicos de extratos obtidos das folhas de M. albicans. Inicialmente, a partir das folhas moídas de M. albicans foi realizada uma extração por percolação com etanol 70% e outra extração por infusão. Ambos os extratos obtidos foram submetidos a uma partição líquido-líquido em acetato de etila/água. A fração orgânica do extrato etanólico (EE70) foi submetida a uma cromatografia de permeação em gel de Sephadex LH-20 eluida com etanol 95% para separação de seus componentes. Ao todo, foram coletadas 60 frações que, após análise em CLAEDAD, foram reunidas de acordo com semelhança. As frações que apresentaram cromatogramas com picos isolados foram novamente submetidas a cromatografia de permeação em gel, para purificação das substâncias. Foram isoladas e purificadas três substâncias. Os espectros de UV apresentados para cada uma destas substâncias foram comparados com o espectro de UV do ácido elágico, indicando que estas substâncias isoladas são possíveis derivadas do ácido elágico.
Popularly known as “canela-de-velho”, Miconia albicans is used as an anti-inflammatory, being sold freely in the form of ointments, capsules or even dried or crushed leaves. However, studies of this species are scarce. Therefore, the objective of the present study was to isolate, purify and identify possible chemical markers of extracts obtained from M. albicans leaves. Initially, a percolation with ethanol 70% and another extraction by infusion was performed from the crushed leaves of M. albicans. Both extracts were collected to a liquid-liquid partition in ethyl acetate / water. The organic fraction of the ethanol extract (EE70) was subjected to a gel permeation chromatography on a Sephadex LH-20 eluted with ethanol 95% to separate its components. Altogether, 60 fractions were collected which, after analysis in HPLC-DAD, were pooled according to similarity. The fractions that presented chromatograms with isolated peaks were again submitted to gel permeation chromatography, for purification of the substances. Three substances were isolated and purified. The UV spectra presented for each of these substances were compared to the UV spectrum of ellagic acid, indicating that these isolated substances are possible ellagic acid derivates.
Popularly known as “canela-de-velho”, Miconia albicans is used as an anti-inflammatory, being sold freely in the form of ointments, capsules or even dried or crushed leaves. However, studies of this species are scarce. Therefore, the objective of the present study was to isolate, purify and identify possible chemical markers of extracts obtained from M. albicans leaves. Initially, a percolation with ethanol 70% and another extraction by infusion was performed from the crushed leaves of M. albicans. Both extracts were collected to a liquid-liquid partition in ethyl acetate / water. The organic fraction of the ethanol extract (EE70) was subjected to a gel permeation chromatography on a Sephadex LH-20 eluted with ethanol 95% to separate its components. Altogether, 60 fractions were collected which, after analysis in HPLC-DAD, were pooled according to similarity. The fractions that presented chromatograms with isolated peaks were again submitted to gel permeation chromatography, for purification of the substances. Three substances were isolated and purified. The UV spectra presented for each of these substances were compared to the UV spectrum of ellagic acid, indicating that these isolated substances are possible ellagic acid derivates.
Descrição
Citação
Serafim, A. C. G. Marcadores analíticos para CLAE-DAD de extratos das folhas de Miconia albicans (SW) DC. (Melastomataceae). 2021. 44 f. Trabalho de Conclusão de Curso (Graduação em Farmácia) - Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo, Diadema, 2021.