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Investigation of the substrate specificity of cruzipain, the major cysteine proteinase of Trypanosoma cruzi, through the use of cystatin-derived substrates and inhibitors
(Portland Press Ltd, 1996-02-01)
A panel of intramolecularly quenched fluorogenic substrates containing the conserved QVVA and LVG inhibitory sequences of cystatin inhibitors was used to describe the specificity of the major cysteine proteinase of Trypanosoma ...
A glimpse on biological activities of tellurium compounds
(Academia Brasileira de Ciências, 2009-09-01)
Tellurium is a rare element which has been regarded as a toxic, non-essential trace element and its biological role is not clearly established to date. Besides of that, the biological effects of elemental tellurium and ...
Analysis of catalytic properties of tripeptidyl peptidase I (TTP-I), a serine carboxyl lysosomal protease, and its detection in tissue extracts using selective FRET peptide substrate
(Elsevier Science Inc, 2016)
Tripeptidyl peptidase I (TPP-I), also named ceroid lipofuscinosis 2 protease (CLN2p), is a serine carboxyl lysosomal protease involved in neurodegenerative diseases, and has both tripeptidyl amino- and endopeptidase ...
DETERMINANTS OF THE UNUSUAL CLEAVAGE SPECIFICITY OF LYSYL-BRADYKININ-RELEASING KALLIKREINS
(Portland Press, 1995-02-15)
Kinetic data for the hydrolysis by human tissue kallikrein of fluorogenic peptides with o-aminobenzoyl-Phe-Arg (Abz-FR) as the acyl group and different leaving groups demonstrate that interactions with the S'(1), S'(2), ...
Positively Selected Sites at HCMV gB Furin Processing Region and Their Effects in Cleavage Efficiency
(Frontiers Media Sa, 2017)
Human cytomegalovirus is a ubiquitous infectious agent that affects mainly immunosuppressed, fetuses, and newborns. The virus has several polymorphic regions, in particular in the envelope glycoproteins. The UL55 gene ...
SYNTHESIS AND KINETIC-PARAMETERS OF HYDROLYSIS BY TRYPSIN OF SOME ACYL-ARGINYL-PARA-NITROANILIDES AND PEPTIDES CONTAINING ARGINYL-PARA-NITROANILIDE
(Assoc Bras Divulg Cientifica, 1985-01-01)
Design of kallidin-releasing tissue kallikrein inhibitors based on the specificities of the enzyme's binding subsites
(Portland Press, 1997-04-01)
The tissue kallikrein inhibitors reported in the present work were derived by selectively replacing residues in N-alpha-substituted arginine- or phenylalanine-pNA (where pNA is p-nitroanilide), and in peptide substrates ...
The use of Fluorescence Resonance Energy Transfer (FRET) peptidesfor measurement of clinically important proteolytic enzymes
(Academia Brasileira de Ciências, 2009-09-01)
Proteolytic enzymes have a fundamental role in many biological processes and are associated with multiple pathological conditions. Therefore, targeting these enzymes may be important for a better understanding of their ...
Met-lys-bradykinin-ser, the kinin released from human kininogen by human pepsin
(Elsevier B.V., 1996-05-01)
This study was carried out to show the site in kininogen, using synthetic substrates, that is cleaved by a purified human pepsin component with a molecular weight of 35 000 daltons. The study used 4 (four) intramolecularly ...
EVALUATION OF THE EXTENT OF THE BINDING-SITE IN HUMAN TISSUE KALLIKREIN BY SYNTHETIC SUBSTRATES WITH SEQUENCES OF HUMAN KININOGEN FRAGMENTS
(Portland Press, 1995-11-15)
We have synthesized internally quenched peptides spanning the Met(379)-Lys(380) Or Arg(389)-Ser(390) bonds of human kininogen (hkng) that flank lysyl-bradykinin and have studied the kinetics of their hydrolysis by human ...