CTPS forms the cytoophidium in zebrafish

CTPS forms the cytoophidium in zebrafish

Author Chang, Chia-Chun Google Scholar
Keppeke, Gerson Dierley Autor UNIFESP Google Scholar
Antos, Christopher L. Google Scholar
Peng, Min Google Scholar
Andrade, Luis Eduardo Coelho Autor UNIFESP Google Scholar
Sung, Li-Ying Google Scholar
Liu, Ji-Long Google Scholar
Abstract Cytidine triphosphate synthase (CTPS) catalyzes the rate-limiting step of de novo CTP biosynthesis. An intracellular structure of CTPS, the cytoophidium, has been found in many organisms including prokaryotes and eukaryotes. Formation of the cytoophidium has been suggested to regulate the activity and stability of CTPS and may participate in certain physiological events. Herein, we demonstrate that both CTPS1a and CTPS1b in zebrafish are able to form the cytoophidium in cultured cells. A point mutation, H355A, abrogates cytoophidium assembly of zebrafish CTPS1a and CTPS1b. In addition, we show the presence of CTPS cytoophidia in multiple tissues of larval and adult fish under normal conditions, while treatment with a CTPS inhibitor 6-diazo-5-oxo-l-norleucine (DON) can induce more cytoophidia in some tissues. Our findings reveal that forming the CTPS cytoophidium is a natural phenomenon of zebrafish and provide valuable information for future research on the physiological importance of this intracellular structure in vertebrates.
Keywords CTPS
xmlui.dri2xhtml.METS-1.0.item-coverage New York
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number 2017/20745-1
Date 2021-08-15
Published in PMID: 34129847
CHANG, Chia-Chun; KEPPEKE, Gerson Dierley; ANTOS, Christopher L.; PENG, Min; ANDRADE, Luis Eduardo Coelho; SUNG, Li-Ying; LIU, Ji-Long. CTPS forms the cytoophidium in zebrafish. Experimental Cell Research, v. 405, n. 2, p. 112684, 2021.
ISSN 0014-4827 (Sherpa/Romeo)
Publisher Andras Simon
Extent 112684
Origin https://www.sciencedirect.com/science/article/abs/pii/S0014482721002160?via%3Dihub
Access rights Closed access
Type Article
URI https://repositorio.unifesp.br/handle/11600/61362

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