Análise Multielementar de Tecidos Biológicos do Fígado de Ratos Wistar Machos Adultos Submetidos à Superexposição de Manganês (Mn)
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Data
2021-02-26
Autores
Gonçalves, Ruan Ataulo Muniz [UNIFESP]
Orientadores
Barbosa, Renata de Faria [UNIFESP]
Tipo
Trabalho de conclusão de curso de graduação
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Resumo
O manganês (Mn) é um elemento considerado essencial ao desenvolvimento corporal de todos os mamíferos, todavia, em níveis elevados, podem se tornar tóxicos (afetando as partes motoras e psíquicas). Os animais foram previamente intoxicados com duas doses diferentes de uma solução de Mn com período de exposição de 30(trinta) dias. Ratos machos adultos da linhagem Wistar com 83 dias de vida (n=24) foram distribuídos em três grupos experimentais (n = 8/grupo): C (grupo controle, recebeu apenas o veículo solução salina a 0,9%); Mn1 (recebeu cloreto de Mn, MnCl2, na dose de 5 mg/Kg de massa corpórea, diluído em solução salina a 0,9%) e Mn2 (recebeu cloreto de Mn, MnCl2, na dose de 15 mg/Kg de massa corpórea, diluído em solução salina a 0,9%). Assim, o objetivo desse estudo foi avaliar a absorção do Mn no fígado, e também verificar se há correlação entre a superexposição ao Mn com os níveis de outros metais presentes no fígado desses animais. As medições foram realizadas em duplicatas usando a técnica de Fluorescência de Raios X por Reflexão Total (TXRF) através do espectrômetro S2 PicoFox – Bruker, disponível no Instituto do Mar, UNIFESP/BS. Para as análises por TXRF, as amostras sólidas precisam ser suspensas em uma solução. Então, à 100 mg de massa seca e macerada do tecido do fígado adicionou-se 1,0 mL de triton (1%), seguida de 1,0 mL de água deionizada. Foi também adicionado o padrão interno: gálio, a fim de atender a metodologia para quantificação. No fígado dos ratos estudados encontramos Na, Cl, K, Ca, Cr, Mn, Fe, Cu, Zn, Se, Rb e Sr. A estatística usada foi o Anova One-Way seguido do teste Bonferroni com nível de significância de 0,05.
Analisando os níveis de Mn no fígado, observamos que a intoxicação via manganês resultou em um acréscimo no fígado deste elemento. Além disso, a superexposição ao manganês gerou também um aumento estatisticamente significativo nos níveis de cálcio, sódio e zinco no grupo Mn2, intoxicados com 15mg/kg de manganês, em relação ao grupo controle.
Manganese (Mn) is an element considered essential to the body development of all mammals, however, at high levels, they can become toxic (affecting the motor and psychic parts). The animals were previously intoxicated with two different doses of a Mn solution with an exposure period of 30 (thirty) days. Adult male rats of the Wistar lineage (n = 24) were divided into three experimental groups (n = 8 / group): C (control group, received only the 0.9% saline vehicle); Mn1 (received Mn chloride, MnCl2, at a dose of 5 mg / kg body weight, diluted in 0.9% saline) and Mn2 (received Mn chloride, MnCl2, at a dose of 15 mg / kg body weight , diluted in 0.9% saline). Thus, the objective of this study was to evaluate the absorption of Mn in the liver, and also to verify whether there is a correlation between overexposure to Mn and the levels of other metals present in the liver of these animals. The measurements were performed in duplicates using the Total Reflection X-Ray Fluorescence (TXRF) technique using the S2 PicoFox - Bruker spectrometer, available at Instituto do Mar, UNIFESP / BS. For TXRF analysis, solid samples need to be suspended in a solution. Then, to 100 mg of dry and macerated mass of liver tissue, 1.0 ml of triton (1%) was added, followed by 1.0 ml of deionized water. The internal standard was also added: gallium, in order to meet the methodology for quantification. In the liver of the studied rats we found Na, Cl, K, Ca, Cr, Mn, Fe, Cu, Zn, Se, Rb and Mr. The statistic used was the Anova One-Way followed by the Bonferroni test with a significance level of 0, 05. Analyzing the levels of Mn in the liver, we observed that intoxication via manganese resulted in an increase in the liver of this element. In addition, overexposure to manganese also generated a statistically significant increase in the levels of calcium, sodium and zinc in the Mn2 group, intoxicated with 15mg / kg of manganese, compared to the control group.
Manganese (Mn) is an element considered essential to the body development of all mammals, however, at high levels, they can become toxic (affecting the motor and psychic parts). The animals were previously intoxicated with two different doses of a Mn solution with an exposure period of 30 (thirty) days. Adult male rats of the Wistar lineage (n = 24) were divided into three experimental groups (n = 8 / group): C (control group, received only the 0.9% saline vehicle); Mn1 (received Mn chloride, MnCl2, at a dose of 5 mg / kg body weight, diluted in 0.9% saline) and Mn2 (received Mn chloride, MnCl2, at a dose of 15 mg / kg body weight , diluted in 0.9% saline). Thus, the objective of this study was to evaluate the absorption of Mn in the liver, and also to verify whether there is a correlation between overexposure to Mn and the levels of other metals present in the liver of these animals. The measurements were performed in duplicates using the Total Reflection X-Ray Fluorescence (TXRF) technique using the S2 PicoFox - Bruker spectrometer, available at Instituto do Mar, UNIFESP / BS. For TXRF analysis, solid samples need to be suspended in a solution. Then, to 100 mg of dry and macerated mass of liver tissue, 1.0 ml of triton (1%) was added, followed by 1.0 ml of deionized water. The internal standard was also added: gallium, in order to meet the methodology for quantification. In the liver of the studied rats we found Na, Cl, K, Ca, Cr, Mn, Fe, Cu, Zn, Se, Rb and Mr. The statistic used was the Anova One-Way followed by the Bonferroni test with a significance level of 0, 05. Analyzing the levels of Mn in the liver, we observed that intoxication via manganese resulted in an increase in the liver of this element. In addition, overexposure to manganese also generated a statistically significant increase in the levels of calcium, sodium and zinc in the Mn2 group, intoxicated with 15mg / kg of manganese, compared to the control group.
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Citação
GONÇALVES, Ruan Ataulo Muniz. Análise Multielementar de Tecidos Biológicos do Fígado de Ratos Wistar Machos Adultos Submetidos à Superexposição de Manganês (Mn). 2021. 30f.
Trabalho de conclusão de curso de graduação (Bacharelado Interdisciplinar em Ciência e Tecnologia do Mar) - Instituto do Mar, Universidade Federal de São Paulo, Santos, 2021.