Quorum Sensing e Staphylococcus capitis
Nenhuma Miniatura disponível
Data
2021-02-12
Autores
Gomes, Jeneffer Garcia [UNIFESP]
Orientadores
Lopes, Patrícia Santos [UNIFESP]
Tipo
Trabalho de conclusão de curso de graduação
Título da Revista
ISSN da Revista
Título de Volume
Resumo
O objeto deste trabalho aborda o tema quorum sensing e sua relação com a
bactéria Staphylococcus capitis. Quorum sensing baseia-se na comunicação entre
microrganismos, possui relação com a expressão de genes celulares, tem a
capacidade de coordenação de características e processos celulares e está
vinculada à bioluminescência, produção de antibióticos, fatores de virulência e a
formação de biofilmes. Acredita-se que a Staphylococcus capitis, uma espécie de
bactéria encontrada na microbiota na pele humana, também utiliza desse
mecanismo. A comunicação entre as bactérias ocorre através de moléculas
sinalizadoras ou autoindutores, elas desempenham papéis fundamentais para
ocorrer esse processo. Para identificação desses metabólitos, métodos como
filtração, extração e CLAE-DAD foram utilizados. Os extratos obtidos foram
testados frente ao Staphylococcus aureus avaliar o comportamento de
Staphylococcus capitis com outra bactéria, observando se esses metabólitos
apresentavam a capacidade de inibir o ciclo de vida da mesma. Os resultados
mostraram nas análises em CLAE-DAD que o extrato de S. capitis não apresentou
substâncias presentes nos comprimentos de onda analisados (254 e 360 nm),
além daquelas já presentes no meio de cultura e o extrato de S.capitis também
não foi capaz de inibir o crescimento de S.aureus. Os resultados, apesar de
negativos, indicam que os métodos empregados não estão adequados para a
avaliação da atividade de Quorum sensing, sendo necessário a readequação dos
mesmos.
The object of this work addresses the theme quorum sensing and its relationship with the bacterium Staphylococcus capitis. Quorum sensing is based on communication between microorganisms, is related to the expression of cellular genes, has the ability to coordinate cellular characteristics and processes and is linked to bioluminescence, antibiotic production, virulence factors and biofilm formation. Staphylococcus capitis, a species of bacteria found in the microbiota on human skin, is also believed to use this mechanism. Communication between bacteria occurs through signaling molecules or self-inducing, they play fundamental roles in the process. To identify these metabolites, methods such as filtration, extraction and HPLC-DAD were used. The extracts obtained were tested against Staphylococcus aureus to evaluate the behavior of Staphylococcus capitis with another bacterium, observing whether these metabolites had the ability to inhibit the life cycle of the same. The results showed in the analyses in HPLC-DAD that the extract of S. capitis did not present substances present in the wavelengths analyzed (254 and 360 nm), in addition to those already present in the culture medium and the extract of S.capitis was also not able to inhibit the growth of S.aureus. The results, although negative, indicate that the methods employed are not adequate for the evaluation of quorum sensing activity, and it is necessary to refit them.
The object of this work addresses the theme quorum sensing and its relationship with the bacterium Staphylococcus capitis. Quorum sensing is based on communication between microorganisms, is related to the expression of cellular genes, has the ability to coordinate cellular characteristics and processes and is linked to bioluminescence, antibiotic production, virulence factors and biofilm formation. Staphylococcus capitis, a species of bacteria found in the microbiota on human skin, is also believed to use this mechanism. Communication between bacteria occurs through signaling molecules or self-inducing, they play fundamental roles in the process. To identify these metabolites, methods such as filtration, extraction and HPLC-DAD were used. The extracts obtained were tested against Staphylococcus aureus to evaluate the behavior of Staphylococcus capitis with another bacterium, observing whether these metabolites had the ability to inhibit the life cycle of the same. The results showed in the analyses in HPLC-DAD that the extract of S. capitis did not present substances present in the wavelengths analyzed (254 and 360 nm), in addition to those already present in the culture medium and the extract of S.capitis was also not able to inhibit the growth of S.aureus. The results, although negative, indicate that the methods employed are not adequate for the evaluation of quorum sensing activity, and it is necessary to refit them.