Efeito do extrato padronizado de Ginkgo biloba (EGb) sobre neuropeptídeos hipotalâmicos envolvidos na regulação da homeostase energética de ratas ovariectomizadas
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Data
2020
Autores
Ático, Esther Milani [UNIFESP]
Orientadores
Telles, Mônica Marques [UNIFESP]
Tipo
Trabalho de conclusão de curso de graduação
Título da Revista
ISSN da Revista
Título de Volume
Resumo
A menopausa é um processo fisiológico natural do corpo feminino que ocorre
em torno dos 50 anos de vida e é marcado por diversas e profundas alterações em
todos os sistemas do organismo. A carência de estrógeno é considerada a principal
causa dos sintomas. Onde diversas alterações endógenas resultam em uma
predisposição para o ganho de massa corporal, além de mudanças no metabolismo
que favorecem o estabelecimento de uma consequência importante da menopausa,
a obesidade. O desenvolvimento da obesidade, a qual tem como característica um
grande acúmulo de tecido adiposo, também induz o estabelecimento de um perfil
inflamação crônico dos tecidos periféricos e do sistema nervoso central (SNC),
condição que promove vários riscos para uma série de outras desordens
metabólicas como hipertensão e diabetes. Promover seu controle é, portanto, de
extrema importância, e novas terapias farmacológicas, baseadas em produtos
naturais, poderiam ser uma alternativa para este fim. Desse modo, visando
compreender o efeito do tratamento com o extrato padronizado de Ginkgo biloba
(EGb) sobre neuropeptídios envolvidos na homeostase energética em fêmeas
ovariectomizadas, ratas de dois meses de idade foram submetidas à cirurgia de
ovariectomia (OVX) ou falsa ovariectomia (FOVX). 60 dias após a cirurgia, as ratas
dos grupos OGb e FGb receberam gavagem diária do tratamento com 500 mg/kg de
EGb, enquanto as fêmeas OS e FS receberam gavagem diária com solução salina a
0,9% como veículo, durante 14 dias. As ratas ovariectomizadas apresentaram um
aumento no ganho de massa corporal após a realização da cirurgia, que condiz com
a alteração corporal que ocorre na menopausa. Porém, não houve diferença
estatística na expressão dos neuropepetídeos NPY e POMC no hipotálamo lateral
(HL) após o tratamento com EGb. Desta forma, estudos mais abrangentes e
aprofundados se fazem necessário para a elucidação dos mecanismos de ação do
extrato sobre os diferentes núcleos hipotalâmicos relacionados a regulação da
homeostase energética.
Menopause is a natural physiological process of the female body that happens around of 50 years and it is marked by several and profound changes in all body systems. Estrogen deficiency is considered the main cause of symptoms, where several endogenous alterations results in a predisposition to the body mass gain, in addition to changes in metabolism that favor the establishment of an important menopause consequence, obesity. The development of obesity, which is characterized by a large accumulation of adipose tissue, also induces the establishment of a chronic inflammation profile of the peripheral tissues and the central nervous system (CNS), a condition that promotes several risks to a series of others metabolic disorders, such as hypertension and diabetes. Promoting its control is, therefore, extremely important and new pharmacological therapies, focusing in natural products, might be an alternative for this purpose. Thus, in order to understand the treatment effect of the standardized extract of Ginkgo biloba (EGb) on neuropeptides involved in energy homeostasis in ovariectomized rats, two-monthold female rats were submitted to ovariectomy (OVX) or false ovariectomy (FOVX) surgery. 60 days after surgery, the rats of the OGb and FGb groups received d a daily gavage with 500 mg/Kg of EGb, while the OS and FS groups received a daily gavage with 0.9% saline for 14 days. The ovariectomized rats showed an increased body mass gain after the surgery, which is consistent with the body change that occurs with menopause. However, there was no significant difference in neuropepetides NPY and POMC expression in the lateral hypothalamus (HL) of these animals after the EGb treatment. Thereby, more comprehensive and in-depth studies are needed to elucidate the mechanisms of action that the extract on different hypothalamic nuclei related with the regulation of energy homeostasis.
Menopause is a natural physiological process of the female body that happens around of 50 years and it is marked by several and profound changes in all body systems. Estrogen deficiency is considered the main cause of symptoms, where several endogenous alterations results in a predisposition to the body mass gain, in addition to changes in metabolism that favor the establishment of an important menopause consequence, obesity. The development of obesity, which is characterized by a large accumulation of adipose tissue, also induces the establishment of a chronic inflammation profile of the peripheral tissues and the central nervous system (CNS), a condition that promotes several risks to a series of others metabolic disorders, such as hypertension and diabetes. Promoting its control is, therefore, extremely important and new pharmacological therapies, focusing in natural products, might be an alternative for this purpose. Thus, in order to understand the treatment effect of the standardized extract of Ginkgo biloba (EGb) on neuropeptides involved in energy homeostasis in ovariectomized rats, two-monthold female rats were submitted to ovariectomy (OVX) or false ovariectomy (FOVX) surgery. 60 days after surgery, the rats of the OGb and FGb groups received d a daily gavage with 500 mg/Kg of EGb, while the OS and FS groups received a daily gavage with 0.9% saline for 14 days. The ovariectomized rats showed an increased body mass gain after the surgery, which is consistent with the body change that occurs with menopause. However, there was no significant difference in neuropepetides NPY and POMC expression in the lateral hypothalamus (HL) of these animals after the EGb treatment. Thereby, more comprehensive and in-depth studies are needed to elucidate the mechanisms of action that the extract on different hypothalamic nuclei related with the regulation of energy homeostasis.