Effects of transcutaneous electrical nerve stimulation on fetal and placental development in an experimental model of placental insufficiency

Effects of transcutaneous electrical nerve stimulation on fetal and placental development in an experimental model of placental insufficiency

Author Guimaraes, Camila S. O. Google Scholar
Gomes, Bruno B. F. Google Scholar
Oliveira, Rafael A. Google Scholar
Yamamoto, Leandro R. Google Scholar
Rocha, Laura P. Google Scholar
Gloria, Maria A. Autor UNIFESP Google Scholar
Machado, Juliana R. Google Scholar
Camara, Niels O. S. Autor UNIFESP Google Scholar
Reis, Marlene A. Google Scholar
Correa, Rosana R. M. Google Scholar
Abstract Objective: To elucidate the effects of transcutaneous electrical nerve stimulation (TENS) in pregnancies with placental insufficiency.Methods: Pregnant rats were subjected to uterine artery ligation and to TENS according to the following groups: ligated stimulated (LS); ligated non-stimulated (LN), control stimulated (CS); and control non-stimulated (CN). Fetal external measurements, such as crown-rump length (CRL), fronto-occipital distance (FOD), thoracic ventral-dorsal (TVDD) and abdominal ventral-dorsal (AVDD) distances were analyzed together with the area occupied by fetal internal organs. Glucose transporter 1 (GLUT-1) expression was evaluated by immunohistochemistry in fetal organs. Thickness of junctional, labyrinth and intermediate placental zones was analyzed by morphometric evaluation in HE-stained slides, and placental hypoxia-inducible factor 1 alfa expression was measured by real-time polymerase chain reaction.Results: In LN and CS groups compared to the CN group, CRL was reduced (27.51/28.95 versus 30.16mm), as well as FOD (6.63/6.63 versus 7.36mm), AVDD (7.38/8.00 versus 8.61mm) and TVDD (6.46/6.87 versus 7.23mm). Brain GLUT-1 expression was higher in LS (1.3%) and CS (1.8%). The area occupied by placental vessels in the labyrinth zone (29.673.51 versus 20.83 +/- 7.63) and intermediate zone (26.46 +/- 10.21 versus 10.86 +/- 8.94) was larger in the LS group than in the LN group.Conclusions: Our results suggest a negative effect of TENS on placental development, thus compromising the maintenance of adequate blood flow to the fetus.
Keywords Hypoxia
xmlui.dri2xhtml.METS-1.0.item-coverage Abingdon
Language English
Sponsor Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq - National Counsel of Technological and Scientific Development)
Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES - Coordination for the Improvement of Higher Education Personnel Foundation)
Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG - Research Foundation of the State of Minas Gerais)
Fundacao de Ensino e Pesquisa de Uberaba (FUNEPU - Research Foundation of Uberaba)
Date 2016
Published in Journal Of Maternal-Fetal & Neonatal Medicine. Abingdon, v. 29, n. 2, p. 283-289, 2016.
ISSN 1476-7058 (Sherpa/Romeo, impact factor)
Publisher Taylor & Francis Ltd
Extent 283-289
Origin https://doi.org/10.3109/14767058.2014.999034
Access rights Closed access
Type Article
Web of Science ID WOS:000365628300022
URI https://repositorio.unifesp.br/handle/11600/58685

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