Aldefluor protocol to sort keratinocytes stem cells from skin

Show simple item record Ribeiro Noronha, Samuel Marcos [UNIFESP] Gragnani, Alfredo [UNIFESP] Calado Pereira, Thiago Antonio [UNIFESP] Alves Correa, Silvana Aparecida [UNIFESP] Bonucci, Jessica [UNIFESP] Ferreira, Lydia Masako [UNIFESP] 2020-09-01T13:21:21Z 2020-09-01T13:21:21Z 2017
dc.identifier.citation Acta Cirurgica Brasileira. Sao Paulo, v. 32, n. 11, p. 984-994, 2017.
dc.identifier.issn 0102-8650
dc.description.abstract Purpose: To investigate the use Aldefluor r and N, N-Dimethylaminobenzaldehyde (DEAB) to design a protocol to sort keratinocyte stem cells from cultured keratinocytes from burned patients. Methods: Activated Aldefluor r aliquots were prepared and maintained at temperature between 2 to 8 degrees C, or stored at -20 degrees C. Next, the cells were collected following the standard protocol of sample preparation. Results: Best results were obtained with Aldefluor r 1.5 mu l and DEAB 15 mu l for 1 x 10(6) cells, incubated at 37 degrees C for 15 minutes. Flow cytometer range for keratinocyte stem cells separation was evaluated. There were 14.8% of stem cells separated in one sample of keratinocyte culture used to pattern the protocol. After being defined the ideal concentration, the same test pattern was performed in other keratinocyte samples. We observed a final mean of 10.8%. Conclusion: Aldefluor r has been shown as a favorable marking of epidermal keratinocyte stem cells for subsequent separation on a flow cytometer, with detection of 10.8% of epidermal keratinocyte stem cells, in this protocol. en
dc.description.sponsorship FAPESP
dc.format.extent 984-994
dc.language.iso eng
dc.publisher Acta Cirurgica Brasileira
dc.relation.ispartof Acta Cirurgica Brasileira
dc.rights Acesso aberto
dc.subject Keratinocytes en
dc.subject Flow Cytometry en
dc.subject Adult Stem Cells en
dc.title Aldefluor protocol to sort keratinocytes stem cells from skin en
dc.type Artigo
dc.description.affiliation Univ Fed Sao Paulo, UNIFESP, Div Plast Surg, Sao Paulo, Brazil
dc.description.affiliation Univ Fed Sao Paulo, Div Plast Surg, Sao Paulo, SP, Brazil
dc.description.affiliationUnifesp Univ Fed Sao Paulo, UNIFESP, Div Plast Surg, Sao Paulo, Brazil
dc.description.sponsorshipID FAPESP: 2013/10905-0
dc.identifier.file S0102-86502017001100984.pdf
dc.identifier.scielo S0102-86502017001100984
dc.identifier.doi 10.1590/s0102-865020170110000010
dc.description.source Web of Science
dc.identifier.wos WOS:000417846700010
dc.coverage Sao Paulo
dc.citation.volume 32
dc.citation.issue 11


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