Period of Hyperbaric Oxygen Delivery Leads to Different Degrees of Hepatic Ischemia/Reperfusion Injury in Rats

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2016
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Chaves, J. C.
Neto, F. S.
Ikejiri, A. T.
Bertoletto, P. R.
Teruya, R.
Santos Simoes, R. [UNIFESP]
Tikazawa, E. H.
Liu, J. B.
Carrara, F. L.
Taha, M. O. [UNIFESP]
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Background. This study evaluated the morphology of the rat liver when hyperbaric oxygen (HBO) was used at various stages of ischemia and reperfusion. Methods. Thirty-two male Wistar rats, subjected to 30 minutes of hepatic ischemia and 30 minutes of reperfusion, were randomly assigned as follows: GIR (n = 8), control without HBO; GHBO/I (n = 8), in which HBO was applied only during ischemia; GHBO/R (n = 8), HBO only during reperfusion; and GHBO/IR (n = 8), HBO during both ischemia and reperfusion. Feasibility scores of hepatocytes were determined by assessing 8 items related to liver injury. Results. The histologic injury score of the hepatic specimens was significantly lower in the GHBO/I group (79.0 +/- 0.1) compared with the GIR group (135.0 +/- 0.1). HBO was not effective when applied during reperfusion (GHBO/R, 151.3 +/- 0.1) or during the ischemia plus reperfusion period (GHBO/IR, 131.0 +/- 0.1). The sum was significantly higher (P < .05) in HBO-treated animals during the reperfusion period (ie, in the GHBO/R group compared with any of the other groups). Conclusions. A favorable effect was obtained when HBO was administered early during ischemia. HBO given in later periods of reperfusion was associated with a more severe sum index percentage of liver damage.
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Transplantation Proceedings. New York, v. 48, n. 2, p. 516-520, 2016.
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