Human skin penetration and local effects of topical nano zinc oxide after occlusion and barrier impairment

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dc.contributor.author Leite-Silva, V. R. [UNIFESP]
dc.contributor.author Sanchez, W. Y.
dc.contributor.author Studier, H.
dc.contributor.author Liu, D. C.
dc.contributor.author Mohammed, Y. H.
dc.contributor.author Holmes, A. M.
dc.contributor.author Ryan, E. M.
dc.contributor.author Haridass, I. N.
dc.contributor.author Chandrasekaran, N. C.
dc.contributor.author Becker, W.
dc.contributor.author Grice, J. E.
dc.contributor.author Benson, H. A. E.
dc.contributor.author Roberts, M. S.
dc.date.accessioned 2020-08-14T13:44:28Z
dc.date.available 2020-08-14T13:44:28Z
dc.date.issued 2016
dc.identifier http://dx.doi.org/10.1016/j.ejpb.2016.04.022
dc.identifier.citation European Journal Of Pharmaceutics And Biopharmaceutics. Amsterdam, v. 104, p. 140-147, 2016.
dc.identifier.issn 0939-6411
dc.identifier.uri https://repositorio.unifesp.br/handle/11600/57683
dc.description.abstract Public health concerns continue to exist over the safety of zinc oxide nanoparticles that are commonly used in sunscreen formulations. In this work, we assessed the effects of two conditions which may be encountered in everyday sunscreen use, occlusion and a compromised skin barrier, on the penetration and local toxicity of two topically applied zinc oxide nanoparticle products. Caprylic/capric triglyceride (CCT) suspensions of commercially used zinc oxide nanoparticles, either uncoated or with a silane coating, were applied to intact and barrier impaired skin of volunteers, without and with occlusion for a period of six hours. The exposure time was chosen to simulate normal in-use conditions. Multiphoton tomography with fluorescence lifetime imaging was used to noninvasively assess zinc oxide penetration and cellular metabolic changes that could be indicative of toxicity. We found that zinc oxide nanoparticles did not penetrate into the viable epidermis of intact or barrier impaired skin of volunteers, without or with occlusion. We also observed no apparent toxicity in the viable epidermis below the application sites. These findings were validated by ex vivo human skin studies in which zinc penetration was assessed by multiphoton tomography with fluorescence lifetime imaging as well as Zinpyr-1 staining and toxicity was assessed by MTS assays in zinc oxide treated skin cryosections. In conclusion, applications of zinc oxide nanoparticles under occlusive in-use conditions to volunteers are not associated with any measurable zinc oxide penetration into, or local toxicity in the viable epidermis below the application site. (C) 2016 Elsevier B.V. All rights reserved. en
dc.description.sponsorship National Health & Medical Research Council of Australia
dc.format.extent 140-147
dc.language.iso eng
dc.publisher Elsevier Science Bv
dc.relation.ispartof European Journal Of Pharmaceutics And Biopharmaceutics
dc.rights Acesso restrito
dc.subject Zinc oxide nanoparticles en
dc.subject Skin penetration en
dc.subject Barrier impairment en
dc.subject Occlusion en
dc.subject Toxicity en
dc.subject Sunscreens en
dc.subject Safety en
dc.subject In-use application en
dc.title Human skin penetration and local effects of topical nano zinc oxide after occlusion and barrier impairment en
dc.type Artigo
dc.description.affiliation Univ Fed Sao Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Diadema, SP, Brazil
dc.description.affiliation Univ Queensland, Translat Res Inst, Sch Med, Therapeut Res Ctr, Brisbane, Qld 4102, Australia
dc.description.affiliation Univ S Australia, Sch Pharm & Med Sci, Adelaide, SA 5001, Australia
dc.description.affiliation Becker & Hickl GmbH, Nahmitzer Damm 30, D-12277 Berlin, Germany
dc.description.affiliation Curtin Univ, CHIRI, Sch Pharm, GPO Box U1987, Perth, WA, Australia
dc.description.affiliationUnifesp Univ Fed Sao Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Diadema, SP, Brazil
dc.identifier.doi 10.1016/j.ejpb.2016.04.022
dc.description.source Web of Science
dc.identifier.wos WOS:000378662400015
dc.coverage Amsterdam
dc.citation.volume 104



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