Human skin penetration and local effects of topical nano zinc oxide after occlusion and barrier impairment

Human skin penetration and local effects of topical nano zinc oxide after occlusion and barrier impairment

Author Leite-Silva, V. R. Autor UNIFESP Google Scholar
Sanchez, W. Y. Google Scholar
Studier, H. Google Scholar
Liu, D. C. Google Scholar
Mohammed, Y. H. Google Scholar
Holmes, A. M. Google Scholar
Ryan, E. M. Google Scholar
Haridass, I. N. Google Scholar
Chandrasekaran, N. C. Google Scholar
Becker, W. Google Scholar
Grice, J. E. Google Scholar
Benson, H. A. E. Google Scholar
Roberts, M. S. Google Scholar
Abstract Public health concerns continue to exist over the safety of zinc oxide nanoparticles that are commonly used in sunscreen formulations. In this work, we assessed the effects of two conditions which may be encountered in everyday sunscreen use, occlusion and a compromised skin barrier, on the penetration and local toxicity of two topically applied zinc oxide nanoparticle products. Caprylic/capric triglyceride (CCT) suspensions of commercially used zinc oxide nanoparticles, either uncoated or with a silane coating, were applied to intact and barrier impaired skin of volunteers, without and with occlusion for a period of six hours. The exposure time was chosen to simulate normal in-use conditions. Multiphoton tomography with fluorescence lifetime imaging was used to noninvasively assess zinc oxide penetration and cellular metabolic changes that could be indicative of toxicity. We found that zinc oxide nanoparticles did not penetrate into the viable epidermis of intact or barrier impaired skin of volunteers, without or with occlusion. We also observed no apparent toxicity in the viable epidermis below the application sites. These findings were validated by ex vivo human skin studies in which zinc penetration was assessed by multiphoton tomography with fluorescence lifetime imaging as well as Zinpyr-1 staining and toxicity was assessed by MTS assays in zinc oxide treated skin cryosections. In conclusion, applications of zinc oxide nanoparticles under occlusive in-use conditions to volunteers are not associated with any measurable zinc oxide penetration into, or local toxicity in the viable epidermis below the application site. (C) 2016 Elsevier B.V. All rights reserved.
Keywords Zinc oxide nanoparticles
Skin penetration
Barrier impairment
Occlusion
Toxicity
Sunscreens
Safety
In-use application
xmlui.dri2xhtml.METS-1.0.item-coverage Amsterdam
Language English
Sponsor National Health & Medical Research Council of Australia
Date 2016
Published in European Journal Of Pharmaceutics And Biopharmaceutics. Amsterdam, v. 104, p. 140-147, 2016.
ISSN 0939-6411 (Sherpa/Romeo, impact factor)
Publisher Elsevier Science Bv
Extent 140-147
Origin http://dx.doi.org/10.1016/j.ejpb.2016.04.022
Access rights Closed access
Type Article
Web of Science ID WOS:000378662400015
URI https://repositorio.unifesp.br/handle/11600/57683

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