Co-administration of alpha-GalCer analog and TLR4 agonist induces robust CD8(+) T-cell responses to PyCS protein and WT-1 antigen and activates memory-like effector NKT cells

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dc.contributor.author Coelho-dos-Reis, Jordana G.
dc.contributor.author Huang, Jing
dc.contributor.author Tsao, Tiffany
dc.contributor.author Pereira, Felipe V. [UNIFESP]
dc.contributor.author Funakoshi, Ryota
dc.contributor.author Nakajima, Hiroko
dc.contributor.author Sugiyama, Haruo
dc.contributor.author Tsuji, Moriya
dc.date.accessioned 2020-08-14T13:44:25Z
dc.date.available 2020-08-14T13:44:25Z
dc.date.issued 2016
dc.identifier http://dx.doi.org/10.1016/j.clim.2016.04.014
dc.identifier.citation Clinical Immunology. San Diego, v. 168, p. 6-15, 2016.
dc.identifier.issn 1521-6616
dc.identifier.uri https://repositorio.unifesp.br/handle/11600/57659
dc.description.abstract In the present study, the combined adjuvant effect of 7DW8-5, a potent alpha-GalCer-analog, and monophosphoryl lipid A (MPLA), a TLR4 agonist, on the induction of vaccine-induced CD8(+) T-cell responses and protective immunity was evaluated. Mice were immunized with peptides corresponding to the CD8+ T-cell epitopes of a malaria antigen, a circumsporozoite protein of Plasmodium yoelff, and a tumor antigen, a Wilms Tumor antigen-1 (WT-1), together with 7DW8-5 and MPLA, as an adjuvant. These immunization regimens were able to induce higher levels of CD8+ T-cell responses and, ultimately, enhanced levels of protection against malaria and tumor challenges compared to the levels induced by immunization with peptides mixed with 7DW8-5 or MPLA alone. Co-administration of 7DW8-5 and MPLA induces activation of memory-like effector natural killer T (NKT) cells, i.e. CD44(+) CD62L(-) NKT cells. Our study indicates that 7DW8-5 greatly enhances important synergistic pathways associated to memory immune responses when co-administered with MPLA, thus rendering this combination of adjuvants a novel vaccine adjuvant formulation. (C) 2016 Elsevier Inc. All rights reserved. en
dc.description.sponsorship NIH
dc.format.extent 6-15
dc.language.iso eng
dc.publisher Academic Press Inc Elsevier Science
dc.relation.ispartof Clinical Immunology
dc.rights Acesso restrito
dc.subject Adjuvant en
dc.subject Glycolipid en
dc.subject NKT cells en
dc.subject CD1d en
dc.subject TLR4 en
dc.subject Malaria vaccine en
dc.subject Cancer vaccine en
dc.subject WT-1 en
dc.subject Circumsporozoite protein en
dc.subject Memory-like effector NKT cells en
dc.title Co-administration of alpha-GalCer analog and TLR4 agonist induces robust CD8(+) T-cell responses to PyCS protein and WT-1 antigen and activates memory-like effector NKT cells en
dc.type Artigo
dc.description.affiliation Rockefeller Univ, Aaron Diamond AIDS Res Ctr, New York, NY 10016 USA
dc.description.affiliation Fundacao Oswaldo Cruz FIOCRUZ, Ctr Pesquisas Rene Rachou, BR-30192 Belo Horizonte, MG, Brazil
dc.description.affiliation Univ Fed Sao Paulo, BR-04021 Sao Paulo, Brazil
dc.description.affiliation Osaka Univ, Grad Sch Med, Dept Canc Immunol, 2-2 Yamadaoka, Suita, Osaka 5650871, Japan
dc.description.affiliation Osaka Univ, Grad Sch Med, Dept Funct Diagnost Sci, 2-2 Yamadaoka, Suita, Osaka 5650871, Japan
dc.description.affiliationUnifesp Univ Fed Sao Paulo, BR-04021 Sao Paulo, Brazil
dc.description.sponsorshipID NIH: AI070258
dc.identifier.doi 10.1016/j.clim.2016.04.014
dc.description.source Web of Science
dc.identifier.wos WOS:000380297600002
dc.coverage San Diego
dc.citation.volume 168



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