Phytochemical screening of the dichloromethaneethanolic extract of Eriosema campestre var. macrophylum roots and its antiproliferative effect on human peripheral blood lymphocytes.

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dc.contributor.author Santos, Michaelle G.
dc.contributor.author Almeida, Valeria G.
dc.contributor.author Avelar-Freitas, Bethania A.
dc.contributor.author Grael, Cristiane F. F.
dc.contributor.author Gregorio, Luiz E. [UNIFESP]
dc.contributor.author Pereira, Wagner F.
dc.contributor.author Brito-Melo, Gustavo E. A.
dc.date.accessioned 2020-08-14T13:44:21Z
dc.date.available 2020-08-14T13:44:21Z
dc.date.issued 2016
dc.identifier http://dx.doi.org/10.1016/j.bjp.2015.08.009
dc.identifier.citation Revista Brasileira De Farmacognosia-Brazilian Journal Of Pharmacognosy. Curitiba-Pr, v. 26, n. 4, p. 464-470, 2016.
dc.identifier.issn 0102-695X
dc.identifier.uri https://repositorio.unifesp.br/handle/11600/57614
dc.description.abstract Eriosema campestre var. macrophylum (Grear) Fortunato, Fabaceae, is a native plant of the Brazilian Cerrado and the decoction of its roots has been used by folk medicine for the therapy of inflammatory diseases. In this study we aimed to investigate the effect of the dichloromethane-ethanolic extract of E. campestre roots on the proliferative response of lymphocytes and to examine the profile of IL-2 production. The effect of dichloromethane-ethanolic extract of E. campestre on the proliferation of phytohemagglutinin-stimulated lymphocytes was evaluated by using flow cytometry and the cell supernatants were assayed for IL-2 concentrations by using an enzyme-linked immunosorbent assay. The phytochemical screening of E. campestre roots was performed to determine the main secondary metabolites through chromogenic and precipitation reactions and by using HPLC-PAD. In addition to the presence of subclasses of flavonoids (flavones and flavonols) in dichloromethaneethanolic extract of E. campestre, we observed that the extract induced a concentration-dependent decrease in IL-2 levels on the supernatant of the cell cultures as well as an antiproliferative effect on T lymphocytes, including CD4+ and CD8+ cells. The anti-inflammatory effects attributed to E. campestre by folk medicine may partly be explained by its antiproliferative action on T lymphocytes. (C) 2016 Sociedade Brasileira de Farmacognosia. Published by Elsevier Editora Ltda. en
dc.description.sponsorship FAPEMIG
dc.description.sponsorship CNPq
dc.description.sponsorship CAPES
dc.format.extent 464-470
dc.language.iso por
dc.publisher Soc Brasileira Farmacognosia
dc.relation.ispartof Revista Brasileira De Farmacognosia-Brazilian Journal Of Pharmacognosy
dc.rights Acesso aberto
dc.subject Eriosema campestre en
dc.subject Flavonoids en
dc.subject Cellular proliferation en
dc.subject IL-2 en
dc.title Phytochemical screening of the dichloromethaneethanolic extract of Eriosema campestre var. macrophylum roots and its antiproliferative effect on human peripheral blood lymphocytes. en
dc.type Artigo
dc.description.affiliation Univ Fed Vales Jequitinhonha & Mucuri, Dept Farm, Lab Imunol, Diamantina, MG, Brazil
dc.description.affiliation Univ Fed Vales Jequitinhonha & Mucuri, Inst Ciencia & Tecnol, Diamantina, MG, Brazil
dc.description.affiliation Univ Fed Vales Jequitinhonha & Mucuri, Lab Farmacognosia, Diamantina, MG, Brazil
dc.description.affiliation Univ Fed Sao Paulo, Lab Ciencias Ambientais Quim & Farmaceut, Diadema, SP, Brazil
dc.description.affiliationUnifesp Univ Fed Sao Paulo, Lab Ciencias Ambientais Quim & Farmaceut, Diadema, SP, Brazil
dc.description.sponsorshipID FAPEMIG: CBB-APQ-00581-11
dc.identifier.file WOS000383732900010.pdf
dc.identifier.scielo S0102-695X2016000400464
dc.identifier.doi 10.1016/j.bjp.2015.08.009
dc.description.source Web of Science
dc.identifier.wos WOS:000383732900010
dc.coverage Curitiba-Pr
dc.citation.volume 26
dc.citation.issue 4



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