Standardization and Prevalence of the Booster Phenomenon: Evaluation Using a Two-Step Skin Test with 43 kDa Glycoprotein in Individuals from an Endemic Region of Paracoccidioidomycosis

Date
2017Author
Marques, Ana Paula da Costa
Oliveira, Sandra Maria do Valle Leone de
Rezende, Grazielli Rocha de
Melo, Dayane Alves de
Fernandes-Fitts, Sonia Maria
Pontes, Elenir Rose Jardim Cury
Bonecini-Almeida, Maria da Gloria
Camargo, Zoilo Pires de [UNIFESP]
Mendes, Rinaldo Poncio
Paniago, Anamaria Mello Miranda
Type
ArtigoISSN
0301-486XIs part of
MycopathologiaDOI
10.1007/s11046-017-0159-zMetadata
Show full item recordAbstract
Background
We estimated the occurrence rate of the booster phenomenon by using an intradermal test with 43 kDa glycoprotein in an endemic area of paracoccidioidomycosis in the central-west region of Brazil.
Methods
Individuals who had a negative result on a survey performed by using an intradermal test with 43 kDa glycoprotein in an endemic area of paracoccidioidomycosis underwent a second intradermal test after 10–15 days to determine the presence or absence of the booster phenomenon. Statistical analyses were performed using the Chi-square test, Chi-square for linear trend test, Student’s t test, and binomial test; p < 0.05 was considered significant.
Results
For the first time, we reported the occurrence of the booster phenomenon to an intradermal reaction caused by 43 kDa glycoprotein at a rate of 5.8–8.4%, depending on the test’s cutoff point. This suggests that a cutoff point should be considered for the booster phenomenon in intradermal tests with 43 kDa glycoprotein: a difference of 6–7 mm between readings according to the first and second tests, depending on the purpose of the evaluation.
Conclusion
The results indicate that the prevalence of paracoccidioidal infection in endemic areas is underestimated, as the booster phenomenon has not been considered in epidemiological surveys for this infection.
Citation
Mycopathologia. Dordrecht, v. 182, n. 43747, p. 809-817, 2017.Keywords
Paracoccidioidic infectionBooster phenomenon
Intradermal tests
Paracoccidioides brasiliensis
Paracoccidioidomycosis
gp43
Sponsorship
Health Department of the Municipality of Jaraguari (Mato Grosso do Sul, Brazil)Post-Graduation Program for Health and Development of the Central-West Region Brazil
Collections
- EPM - Artigos [17709]