Magnetic super-hydrophilic carbon nanotubes/graphene oxide composite as nanocarriers of mesenchymal stem cells: Insights into the time and dose dependences

Magnetic super-hydrophilic carbon nanotubes/graphene oxide composite as nanocarriers of mesenchymal stem cells: Insights into the time and dose dependences

Author Granato, Alessandro Eustaquio Campos Autor UNIFESP Google Scholar
Rodrigues, Bruno V. M. Google Scholar
Rodrigues-Junior, Dorival Mendes Autor UNIFESP Google Scholar
Marciano, Fernanda R. Google Scholar
Lobo, Anderson O. Google Scholar
Porcionatto, Marimélia Aparecida Autor UNIFESP Google Scholar
Abstract Among nanostructured materials, multi-walled carbon nanotubes (MWCNT) have demonstrated great potential for biomedical applications in recent years. After oxygen plasma etching, we can obtain super-hydrophilic MWCNT that contain graphene oxide (GO) at their tips. This material exhibits good dispersion in biological systems due to the presence of polar groups and its excellent magnetic, properties due to metal particle residues from the catalyst that often remain trapped in its walls and tips. Here, we show for the first time a careful biological investigation using magnetic superhydrophilic MWCNT/GO (GCN composites). The objective of this study was to investigate the application of GCN for the in vitro immobilization of mesenchymal stem cells. Our ultimate goal was to develop a system to deliver mesenchymal stem cells to different tissues and organs. We show here that mesenchymal stem cells were able to internalize GCN with a consequent migration when subjected to a magnetic field. The cytotoxicity of GCN was time- and dose-dependent. We also observed that GCN internalization caused changes in the gene expression of the proteins involved in cell adhesion and migration, such as integrins, laminins, and the chemokine CXCL12, as well as its receptor CXCR4. These results suggest that GCN represents a potential new platform for mesenchymal stem cell immobilization at injury sites. (C) 2016 Elsevier B.V. All rights reserved.
xmlui.dri2xhtml.METS-1.0.item-coverage Amsterdam
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Financiadora de Estudos e Projetos (FINEP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Grant number FAPESP: 2011/17877-7
FAPESP: 2011/20345-7
FAPESP: 2012/00652-5
CNPq: 474090/2013-2
CNPq: 404646/2012-3
FINEP: 0113042800
CAPES: 88887.095044/2015-00
FAPESP: 2015/08523-8
Date 2016
Published in Materials Science & Engineering C-Materials For Biological Applications. Amsterdam, v. 67, p. 694-701, 2016.
ISSN 0928-4931 (Sherpa/Romeo, impact factor)
Publisher Elsevier Science Bv
Extent 694-701
Origin http://dx.doi.org/10.1016/j.msec.2016.05.039
Access rights Closed access
Type Article
Web of Science ID WOS:000378952800078
URI https://repositorio.unifesp.br/handle/11600/57095

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