Capillary electrophoresis coupled to contactless conductivity detection for analysis of amino acids of agricultural interest in composting

Show simple item record Cappelini, Luciana Teresa Dias [UNIFESP] Menegoci Eugenio, Patricia de Fatima Gomes Carneiro Leao, Paulo Augusto Alberice, Juliana Vieira Urbaczek, Ana Carolina Assuncao, Nilson Antonio [UNIFESP] Juliano, Luiz [UNIFESP] Carrilho, Emanuel 2020-07-31T12:47:38Z 2020-07-31T12:47:38Z 2016
dc.identifier.citation Electrophoresis. Hoboken, v. 37, n. 19, p. 2449-2457, 2016.
dc.identifier.issn 0173-0835
dc.description.abstract Composting is a sustainable approach to manage animal and vegetal waste generated in the Fundacao Parque Zoologico de Sao Paulo. The resulting compost is often used in ZOO's premises as an organic fertilizer for the production of vegetables, which is further used to feed the animals. The composting product provides many forms of mineral and also amino acids (AA) that are absorbed by plants as nutrients. Since most amino acids absorb only slightly or not at all in the UV wavelengths, we developed a method for the determination of AA of agricultural interest in the composting samples. Due to the complexity of samples, we used ion exchange chromatography for the purification of AA prior to analysis. The proposed CZE-(CD)-D-4 method allowed a separation of the AA in a short analysis time (less than 3.0 min), with great linearity (with R-2 ranging from 0.993 to 0.998). Using a BGE of 10 mmol/L TEA, reduction of high-frequency noise and lower baseline fluctuations were obtained. The LOQ for the five AA were around 35 mu mol/L, and were adequate for our purpose. In addition, the method showed good precision (RSD of peak area and migration time less than 1.55 and 1.16%, respectively). en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent 2449-2457
dc.language.iso eng
dc.publisher Wiley-Blackwell
dc.relation.ispartof Electrophoresis
dc.rights Acesso restrito
dc.subject Amino acids en
dc.subject CE-C4D en
dc.subject Cation exchange chromatography en
dc.subject Composting en
dc.title Capillary electrophoresis coupled to contactless conductivity detection for analysis of amino acids of agricultural interest in composting en
dc.type Artigo
dc.description.affiliation Univ Fed Sao Paulo, UNIFESP, Rua 3 Maio, Sao Paulo, Brazil
dc.description.affiliation Univ Sao Paulo, IQSC, Ave Trabalhador Sao Carlense 400, BR-13566590 Sao Carlos, SP, Brazil
dc.description.affiliation Univ Fed Sao Paulo, Inst Ciencias Ambientais Quim & Farmaceut, UNIFESP, Rua Prof Artur Riedel 275, Diadema, Brazil
dc.description.affiliation Inst Nacl Ciencia & Tecnol Bioanalit INCTBio, Campinas, SP, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo ‐ UNIFESP ‐ Rua 3 de Maio, São Paulo, Brazil
dc.description.affiliationUnifesp Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de Sao Paulo – UNIFESP, Rua Prof. Artur Riedel, 275, Diadema, Brazil
dc.description.sponsorshipID FAPESP: 2014/07037-0
dc.description.sponsorshipID CNPq: 163888/2013-2
dc.description.sponsorshipID CNPq: 482696/2011-7
dc.description.sponsorshipID CNPq: 311323/2011-1
dc.identifier.doi 10.1002/elps.201600302
dc.description.source Web of Science
dc.identifier.wos WOS:000388595400002
dc.coverage Hoboken
dc.citation.volume 37
dc.citation.issue 19


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