HMSH2 and HMSH6 gene expression profiles in colorectal adenocarcinoma in patients up to 50 years of age

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dc.contributor.author Germini, Demetrius
dc.contributor.author Gehrke, Flavia
dc.contributor.author Lira, Daniel
dc.contributor.author Alves, Beatriz
dc.contributor.author Azzalis, Ligia [UNIFESP]
dc.contributor.author Perez, Matheus
dc.contributor.author Fonseca, Fernando [UNIFESP]
dc.contributor.author Waisberg, Jaques
dc.date.accessioned 2020-07-31T12:47:37Z
dc.date.available 2020-07-31T12:47:37Z
dc.date.issued 2016
dc.identifier http://dx.doi.org/10.1016/j.biopha.2016.07.015
dc.identifier.citation Biomedicine & Pharmacotherapy. Paris, v. 83, p. 602-606, 2016.
dc.identifier.issn 0753-3322
dc.identifier.uri https://repositorio.unifesp.br/handle/11600/56949
dc.description.abstract Lynch syndrome, previously called hereditary non-polyposis colorectal cancer (HNPCC), is a major mortality threat. It is an autosomal dominant disease which is caused by a germline mutation in the DNA mismatch repair (MMR), especially in patients aged up to 50 years. Such mutation more frequently occurs in the hMSH2 gene (38-40%) and less frequently in the hMSH6 gene (14-16%). These mutations, when associated with the patient's lifestyle, may reveal a considerable variability in the disease manifestations, such as the degrees of penetrance and clinical aggressiveness. The aim of this study is to analyze the expression of DNA MMR genes, and correlate this expression with the clinical and anatomopathological findings of the neoplasia in patients aged between 39 and 49 years. A total of 45 patients were included: (48.9%) males and (51.1%) females, and they all underwent resection of a colorectal adenocarcinoma. The tissue microarray technique was used to analyze the relative and absolute expression of hMSH2 and hMSH6. Amsterdam II criteria for the diagnosis of HNPCC were obtained from the data provided by medical records and interviews with patients. hMSH2 and hMSH6 was expressed in all patients, which correlated between each other (RHO = 0.669 and p < 0.001) but not to age. There is a positive correlation between the expressions of males (RHO = 0.673 and p = 0.001) and females (RHO = 0.006 and p < 0.001). It is possible to evaluate the expression of MMR genes in embedded anatomopathological samples. Gene expressions correlated between each other and to the sex of the patients, but no difference in relation to age. (C) 2016 Elsevier Masson SAS. All rights reserved. en
dc.format.extent 602-606
dc.language.iso eng
dc.publisher Elsevier France-Editions Scientifiques Medicales Elsevier
dc.relation.ispartof Biomedicine & Pharmacotherapy
dc.rights Acesso restrito
dc.subject Lynch syndrome en
dc.subject DNA mismatch repair en
dc.subject hMSH2 en
dc.subject hMSH6: hereditary non-polyposis colorectal cancer en
dc.subject Colorectal adenocarcinoma en
dc.title HMSH2 and HMSH6 gene expression profiles in colorectal adenocarcinoma in patients up to 50 years of age en
dc.type Artigo
dc.description.affiliation State Civil Servant Hosp IAMSPE, Sao Paulo, Brazil
dc.description.affiliation ABC Med Sch, Oncol Hematol Discipline, Sao Paulo, Brazil
dc.description.affiliation Univ Paulista, Sao Paulo, Brazil
dc.description.affiliation Univ Fed Sao Paulo, Inst Chem & Pharmaceut Sci, Sao Paulo, Brazil
dc.description.affiliation Av Principe de Gales 821, BR-09060650 Santo Andre, SP, Brazil
dc.description.affiliation Av Ibirapuera,981-2 Andar, BR-04029000 Sao Paulo, SP, Brazil
dc.description.affiliationUnifesp Institute of Chemical and Pharmaceutical Sciences, Universidade Federal de São Paulo (UNIFESP), Diadema, São Paulo, Brazil
dc.identifier.doi 10.1016/j.biopha.2016.07.015
dc.description.source Web of Science
dc.identifier.wos WOS:000390433400072
dc.coverage Paris
dc.citation.volume 83



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